About Enhancer
| Enhancer ID: | E_01_048 | |
| Enhancer symbol: | LCT Enhancer | |
| Species: | Human | |
| Position : | chr2:136531405-136531505   |
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| Biosample name: | Caco-2 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 21327791 |
| Enhancer experiment: | Transfection,Luciferase Reporter Assay | |
| Enhancer experiment description: | The LCT enhancer activity has previously been characterized by transfection experiments using a 450 bp fragment. These fragments were cloned at a distance upstream (approximately 3 Kb) of the 1,085 bp LCT promoter in a luciferase reporter gene plasmid, transfected into the intestinal cell line Caco--2 and compared to the full--length 450 bp enhancer construct. |
About Target gene
| Target gene : | LCT(LAC,LPH,LPH1) | |
| Strong evidence: | -- | |
| Less strong evidence: | Transfection,Luciferase Reporter Assay | |
| Target gene experiment description: | Caco--2 cells were transfected with luciferase reporter gene constructs containing either the LCT promoter alone or the LCT promoter with deleted enhancer fragments using either the African LP --14010*C variant (--14010*C; --13910*C) or the European LP --13910*T variant (--14010*G; --13910*T),or the ancestral variants (--14010*G; --13910*C) (Fig. 2). |
About TF
| TF name : | HNF1A(HNF-1A,HNF1,HNF4A,IDDM20,LFB1,MODY3,TCF-1,TCF1)POU2F1(OCT1,OTF1,oct-1B) | |
| TF experiment: | EMSA | |
| TF experiment description: | This indicates that the Oct-1 binding to the -14010*C variant is influenced by the downstream sequence that covers the HNF1a site. The small differences in the Oct-1 binding to the -14010 allelic variants in EMSAs we have found, might be expected to be more pronounced in adult intestine where the pattern of transcription.factor expression and activity allows higher expression than in the Caco-2 cell line. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
