About Enhancer
| Enhancer ID: | E_01_021 | |
| Enhancer symbol: | MYC-ECSE (MYC endometrial carcinoma super-Enhancer) | |
| Species: | Human | |
| Position : | chr8:129543949-129554294   |
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| Biosample name: | Endometrial Carcinoma Cell Lines Ishikawa | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer | |
| Disease: | Endometrial Carcinoma | |
| DO: | DOID:2871 | |
| Mesh: | D016889 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26656844 |
| Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay,3C-qPCR,CRISPR/Cas9,siRNA | |
| Enhancer experiment description: | The endometrial carcinoma peak (chr. 8: 129,543,949–129,554,294) encompasses a 10-kb noncoding region that harbors a super-enhancer,as defined by the H3K27ac ChIP-seq profile of the endometrial carcinoma cell line Ishikawa, which we refer to as MYC-ECSE(MYC endometrial carcinoma super-enhancer). |
About Target gene
| Target gene : | MYC(MRTLC,bHLHe39,c-Myc) | |
| Strong evidence: | 3C | |
| Less strong evidence: | -- | |
| Target gene experiment description: | We performed chromosome conformation capture (3C) assays in A549 and Ishikawa cells and found that MYC-LASE physically interacted with the MYC promoter only in A549 cells and, reciprocally, that MYC-ECSE physically interacted with the MYC promoter only in Ishikawa cells |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | Two distinct focal amplifications of super-enhancers 3′ to MYC in lung adenocarcinoma (MYC-LASE) and endometrial carcinoma (MYC-ECSE) are physically associated with the MYC promoter and correlate with MYC overexpression. |
| Enhancer function experiment: | 3C,ChIP-seq |
| Enhancer function experiment description: |
We performed chromosome conformation capture (3C) assays in A549 and Ishikawa cells and found that MYC-LASE physically interacted with the MYC promoter only in A549 cells and, reciprocally,that MYC-ECSE physically interacted with the MYC promoter only in Ishikawa cells. In addition, tumors with amplification of MYC alone or MYC-LASE or MYC-ECSE alone had higher MYC expression than tumors lacking either amplification. These results suggest that both MYC-LASE and MYC-ECSE drive MYC expression through lineage-specific chromatin loops. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
