About Enhancer
| Enhancer ID: | E_01_015 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr13:73880413-74042621   |
|
| Biosample name: | Esophageal Carcinoma | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer | |
| Disease: | Lung Adenocarcinoma | |
| DO: | DOID:3910 | |
| Mesh: | D000077192 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26656844 |
| Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay,3C-qPCR,CRISPR/Cas9,siRNA | |
| Enhancer experiment description: | The focal amplification on chr13q identified in head and neck squamous cell carcinoma (HNSC) (~110 kb, chr13:73880690-73990596) and esophageal carcinoma (ESCA) (~162 kb, chr13:73880413-74042621) |
About Target gene
| Target gene : | KLF5(BTEB2,CKLF,IKLF) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-seq | |
| Target gene experiment description: | Similarly, the ESCA amplicon also harbors a super-enhancer based on the H3K27ac ChIP-seq profile of esophageal cells and ESCA tumors with this amplicon exhibited a trend towards increased KLF5 expression |
About TF
| TF name : | NFE2L2(HEBP1,IMDDHH,NRF2)CEBPB(C/EBP-beta,IL6DBP,NF-IL6,TCF5) | |
| TF experiment: | ChIP-seq,siRNA,Luciferase Reporter Assay | |
| TF experiment description: | Short interfering RNA (siRNA)-mediated knockdown of NFE2L2 and CEBPB in A549 cells led to a significant reduction in e3-driven luciferase reporter activity as compared to exposure to control siRNAs. Binding of NFE2L2 and CEBPβ to the super-Enhancer was subsequently confirmed by ChIP-seq, with the greatest enrichment at the e3 constituent Enhancer. |
About Function
| Enhancer function : | Copy number gain of the e3 Enhancer region drives MYC overexpression, which contributes to tumorigenic phenotype |
| Enhancer function experiment: | ChIP-seq,CRISPR/Cas9,PCR |
| Enhancer function experiment description: |
Finally, repression of the e3 Enhancer led to a significant decrease in both anchorage-independent and clonogenic growth, suggesting that activity of the e3 Enhancer is critical for the tumorigenicity of lung adenocarcinoma cells. These results suggest that copy number gain of the e3 Enhancer region drives MYC overexpression, which contributes to tumorigenic phenotype. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
