About Enhancer
| Enhancer ID: | E_03221 |
| Species: | Mus musculus |
| Position : | chr3:94294960-94295960   |
| Year: | 2022 |
| Title: | Transcription factor ROR汐 enforces stability of the Th17 cell effector program by binding to a Rorc cis-regulatory element |
| Genome Build: | mm39 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 36243007 |
| Tissue: | small intestine |
| Tissue Ontology ID: | UBERON:0002108 |
| Cell Source: | in vitro 2D2tg Th17 cells |
| Cell Type: | T-helper 17 cell |
| Cell Ontology ID: | CL:0000899 |
| Experiment Type: | Rorc CNS11/+11-kb cis-regulatory element; ROR汐 occupancy; in vivo enhancer-reporter deletion and lineage-maintenance validation; exact 1001-bp CNS11 interval independently validated by PMID 41739921 |
| High Throughput Method: | RNA-seq; ATAC-seq; ROR汐 ChIP-seq; ROR污t ChIP-seq |
| Low Throughput Method: | BAC/transgenic Rorc reporter; +11-kb element deletion reporter; Rora conditional deletion; RORE CRISPR editing; ChIP-qPCR; RT-qPCR; flow cytometry; EAE/peripheral inflammatory challenge |
About Target Gene
| Target gene : | Rorc |
About TF
| TF name : | Rora |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Rora | BMAL1:CLOCK,NPAS2 activates circadian gene expression | reactome | 42 |
| Rora | Circadian Clock | reactome | 49 |
| Rora | HIF-1-alpha transcription factor network | pid | 67 |
| Rora | Nuclear Receptor transcription pathway | reactome | 51 |
| Rora | PPARA activates gene expression | reactome | 113 |
| Rora | RORA activates gene expression | reactome | 27 |
| Rora | Hs_Signaling_of_Hepatocyte_Growth_Factor_Receptor_WP313_79946 | wikipathways | 15 |
| Rora | Hs_Complement_and_Coagulation_Cascades_WP558_90196 | wikipathways | 21 |
Enhancer Associated Network
Overlapping Enhancers (mm39)
12 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02392 | chr3:94294960-94295960 | small intestine | type 17 immune cell | NA |
| E_02393 | chr3:94294960-94295960 | small intestine | type 17 immune cell | NA |
| E_02394 | chr3:94294960-94295960 | small intestine | type 17 immune cell | NA |
| E_03196 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
| E_03197 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
| E_03198 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
| E_03199 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
| E_03219 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
| E_03220 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
| E_03222 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
| E_03223 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
| E_03224 | chr3:94294960-94295960 | small intestine | T-helper 17 cell | NA |
Expression of Target Genes for the Enhancer