About Enhancer
| Enhancer ID: | E_03094 |
| Species: | Mus musculus |
| Position : | chr6:122700483-122702483   |
| Year: | 2014 |
| Title: | Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 25223790 |
| Cell Source: | Embryonic Stem Cell |
| Experiment Type: | Enhancer activity assay; TF-binding/chromatin assay; Endogenous enhancer perturbation |
| High Throughput Method: | ChIP-seq |
| Low Throughput Method: | Luciferase reporter assay; Reporter assay; CRISPR-Cas9 targeted editing/deletion; ChIP-qPCR/ChIP; RT-qPCR/qPCR |
About Target Gene
| Target gene : | Nanog |
About TF
| TF name : | Klf4 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Klf4 | Regulation of nuclear beta catenin signaling and target gene transcription | pid | 80 |
| Klf4 | Synthesis, secretion, and deacylation of Ghrelin | reactome | 17 |
| Klf4 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| Klf4 | Transcriptional regulation of white adipocyte differentiation | reactome | 79 |
| Klf4 | Hs_Role_of_Osx_and_miRNAs_in_tooth_development_WP3971_91525 | wikipathways | 9 |
| Klf4 | Hs_White_fat_cell_differentiation_WP3946_90940 | wikipathways | 30 |
| Klf4 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
13 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_05222 | chr6:122696853-122708211 | NA | NA | NA |
| E_05223 | chr6:122696853-122708211 | NA | NA | NA |
| E_05224 | chr6:122696853-122708211 | NA | NA | NA |
| E_05225 | chr6:122696853-122708211 | NA | NA | NA |
| E_05226 | chr6:122696853-122708211 | NA | NA | NA |
| E_03043 | chr6:122698567-122721126 | embryo | mouse embryonic stem cell | NA |
| E_03095 | chr6:122700483-122702483 | NA | NA | NA |
| E_03096 | chr6:122700483-122702483 | NA | NA | NA |
| E_03097 | chr6:122700483-122702483 | NA | NA | NA |
| E_03117 | chr6:122702344-122703335 | embryo | embryonic stem cell | NA |
| E_03118 | chr6:122702344-122703335 | embryo | embryonic stem cell | NA |
| E_03119 | chr6:122702344-122703335 | epiblast (generic) | epiblast cell | NA |
| E_03120 | chr6:122702344-122703335 | epiblast (generic) | epiblast cell | NA |
Expression of Target Genes for the Enhancer