About Enhancer
| Enhancer ID: | E_02_0987 |
| Species: | mouse |
| Position : | chr15:97977739-97977942   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 19041414 |
| Enhancer experiment: | Luciferase Reporter Assay,EMSA |
| Enhancer experiment description: | Transient transfections of Sox9, Smad3, and T_x0002_R-I(TD) did not increase luciferase activities of pGL3-B plasmids in SW1353 cells (pGL3-B). In pGL3-585E systems, Sox9 enhanced a relative luciferase activity to a level as high as 2.2-fold over the control. Cotransfection of Smad3 augmented a luciferase activity up to 2.3-fold higher level of Sox9-transfected cells. The additional transfection of constitutively active form of T_x0002_R-I(TD) induced an approximately 36% increase of the activity in Sox9- and Smad3-transfected SW1353 cells. Luciferase activities of pGL3-585E were not increased in the absence of Sox9. Note that Smad3 and T_x0002_R-I(TD) synergistically activated the native Col2a1 reporter-mediated transcription in a Sox9-dependent manner. |
About Target gene
| Target gene : | Col2a1(ANFH,AOM,COL11A3,SEDC,STL1) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,EMSA |
| Target gene experiment description: | Purified Sox9 associated with the Col2a1 enhancer probe in EMSA. |
About TF
| TF name : | Sox9(CMD1,CMPD1,SRA1,SRXX2,SRXY10) |
| TF experiment: | EMSA |
| TF experiment description: | Purified Sox9 associated with the Col2a1 enhancer probe in EMSA. The unlabeled competitor decreased the signal of Sox9DNA complex. Supershifted band was observed in the presence of anti-Sox9 antibody. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sox9 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sox9 | TGF_beta_Receptor | netpath | 220 |
| Sox9 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| Sox9 | Hs_Spinal_Cord_Injury_WP2431_87678 | wikipathways | 21 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene