About Enhancer
| Enhancer ID: | E_02_0980 |
| Species: | human |
| Position : | chr15:52315678-52316298   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 20942803 |
| Enhancer experiment: | Luciferase Reporter Assay,PCR |
| Enhancer experiment description: | To address this hypothesis these regions, designated intronic enhancers A and B, respectively, were isolated using PCR and ligated 5? of a heterologous thymidine kinase (TK)-luciferase fusion gene containing TK genomic sequence between ?105 and +51, relative to the transcription start site.Luciferase expression directed by these fusion genes was then analyzed by transient transfection of ?TC-3 cells, an islet beta cell-derived line [31], ?TC-6 cells, an islet alpha cell-derived line [32, 33] and HeLa cells, a cervix-derived cell line. |
About Target gene
| Target gene : | Slc30a8(ZNT8,ZnT-8) |
| Strong evidence: | -- |
| Less strong evidence: | PCR,Luciferase Reporter Assay |
| Target gene experiment description: | Luciferase expression directed by these fusion genes was then analyzed by transient transfection of ?TC-3 cells, an islet beta cell-derived line [31], ?TC-6 cells, an islet alpha cell-derived line [32, 33] and HeLa cells, a cervix-derived cell line. Figure 2 shows that in ?TC-3 cells, but not ?TC-6 or HeLa cells, intronic enhancer A elevated reporter gene expression beyond that driven by the TK-luciferase fusion gene alone, indicating that this region is an islet beta cell-specific enhancer. In contrast, intronic enhancer B had no effect on fusion gene expression in any of the cell lines. |
About TF
| TF name : | Pbx1(2310056B04Rik,D230003C07Rik,Pbx-1) |
| TF experiment: | ChIP,EMSA |
| TF experiment description: | Gel retardation and chromatin immunoprecipitation (ChIP) assays revealed that the islet-enriched transcription factor Pdx-1 binds Enhancer A in vitro and in situ, respectively. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Pbx1 | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
| Pbx1 | Glucocorticoid receptor regulatory network | pid | 85 |
| Pbx1 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene