About Enhancer
| Enhancer ID: | E_02_0956 |
| Species: | mouse |
| Position : | chr1:38469166-38512500   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Acute leukemia |
| Pubmed ID: | 22072553 |
| Enhancer experiment: | Transfection,ChIP-chip,Luciferase Reporter Assay |
| Enhancer experiment description: | Furthermore, a subset of these sites shows enhancer activity in transient transfection assays. ChIP-chip data were analyzed with MA2C 36 and a customized R package.These studies showed that H3 and H4 acetylation and p300/CBP binding is centered on the H/M peaks and is flanked by regions of histone H3K4me1 in a bimodal distribution characteristic of enhancer sequences (Figure 3B). 3To test for potential enhancer (or repressor activity), 22 Hoxa9/Meis1 binding sites as well as 2 control regions randomly selected in the genome where there is no Hoxa9/Meis1 binding were cloned as ~1000-bp fragments. These were inserted into the multiple cloning site of the pTAL-Lucvector,electroporated into myeloblastic K562 cells and were analyzed by Dual Luciferase Reporter Assays (Promega; supplemental Figure 3). |
About Target gene
| Target gene : | Aff3(3222402O04Rik,A730046J16,LAF-4,Laf4) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay |
| Target gene experiment description: | To test for potential enhancer (or repressor activity), 22 Hoxa9/Meis1 binding sites as well as 2 control regions randomly selected in the genome where there is no Hoxa9/Meis1 binding were cloned as ~1000-bp fragments. These were inserted into the multiple cloning site of the pTAL-Lucvector,electroporated into myeloblas- tic K562 cells and were analyzed by Dual Luciferase Reporter Assays (Promega; supplemental Figure 3). |
About TF
| TF name : | Hoxa9(D6a9,Hox-1.7)Meis1(C530044H18Rik,Evi8) |
| TF experiment: | ChIP-qPCR |
| TF experiment description: | Confirmation of selected Hoxa9 and Meis1 binding sites by ChIP and quantitative PCR. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Hoxa9 | TGF_beta_Receptor | netpath | 220 |
| Meis1 | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene