About Enhancer
| Enhancer ID: | E_02_0935 |
| Species: | human |
| Position : | chr12:48369059-48369213   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 23631855 |
| Enhancer experiment: | PCR,ChIP,Luciferase Reporter Assay |
| Enhancer experiment description: | In addition, CTS increased the association between Smad2/3 and the COL2A1 enhancer on chromatin in SW1353 cells (Fig. 3C). In stretched cells, DNA fragment that contained the COL2A1 enhancer was coimmunoprecipitated with Smad2/3, probably via complex for_x0002_mation among Smad2/3-SOX9-the SOX9-binding site, and was amplified by PCR using specific primers for the COL2A1 enhancer. |
About Target gene
| Target gene : | COL2A1(ANFH,AOM,COL11A3,SEDC,STL1) |
| Strong evidence: | -- |
| Less strong evidence: | PCR,ChIP,Luciferase Reporter Assay |
| Target gene experiment description: | Chromatin immunoprecipitation revealed that CTS increased Smad2/3 interaction with the CCN2 promoter and the COL2A1 enhancer. Our results suggest that CTS epigenetically stimulates CCN2 transcription via TGF-?1 release associated with Smad2/3 activation and enhances COL2A1 expression through the complex formation between SOX9 and Smad2/3. |
About TF
| TF name : | SOX9(CMD1,CMPD1,SRA1,SRXX2,SRXY10) |
| TF experiment: | Western blot,ChIP |
| TF experiment description: | In Western blot(WB)analysis,CTS treatment(2h)did not influence total a mounts of endogenous Smad2/3 and SOX9 in each whole cell lysate(B,20 ?g/lane). However,Smad2/3 detected in the nuclear fraction was increased by CTS(C,nucleus,20 ?g/lane). In IP analys is using an anti-SOX9 antibody,CTS increased the association between phosphorylated Smad2/3 and SOX9 in the nuclear fraction derived from stretched SW1353 cells. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| SOX9 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| SOX9 | TGF_beta_Receptor | netpath | 220 |
| SOX9 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| SOX9 | Hs_Spinal_Cord_Injury_WP2431_87678 | wikipathways | 21 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene