About Enhancer
| Enhancer ID: | E_02_0932 |
| Species: | mouse |
| Position : | chr4:115095426-115097426   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 23642367 |
| Enhancer experiment: | ChIP,EMSA |
| Enhancer experiment description: | Several Tal1 enhancers have been identified, but only the +40k enhancer region contains E-box motifs (Ogilvy et al., 2007), potential binding sites for Mesp1 (Figure 3B, left panel). |
About Target gene
| Target gene : | Tal1(SCL,TCL5,bHLHa17,tal-1) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,EMSA |
| Target gene experiment description: | We performed ChIP in day 3 EBs after a prior 24 hr Mesp1 pulse and found that Mesp1 was enriched at this cis -regulatory element (Figure 3B, right panel) with a concurrent upregulation of Tal1 expression (Supplementary Figure S3B).We next performed electrophoretic mobility shift assays to confirm the direct interaction between Mesp1 and the Tal1 +40k Enhancer. |
About TF
| TF name : | Nkx2-5(Csx,Nkx-2.5,Nkx2.5,tinman) |
| TF experiment: | RT-PCR |
| TF experiment description: | At the protein level,the late pulse of Mesp1 elevated the expression of Nkx2.5,a cardiogenic transcription factor,and downstream markers,cTnT,cTnI and sarcomeric ?-actinin. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Nkx2-5 | Physiological factors | reactome | 12 |
| Nkx2-5 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Nkx2-5 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| Nkx2-5 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene