About Enhancer
| Enhancer ID: | E_02_0913 |
| Species: | human |
| Position : | chr7:116374406-116376406   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Melanoma |
| Pubmed ID: | 24443471 |
| Enhancer experiment: | 3C,ChIP |
| Enhancer experiment description: | Chromosome conformation capture (3C)was performed in COLO829 cells in the presence or absence of PLX4032 using the MET +63-kb enhancer as an anchor region. We observed that BRAF inhibition strikingly increased the interaction between the MET +63-kb enhancer and the MET TSS, demonstrating that this lineage-specific enhancer undergoes inducible chromatin looping in response to oncogene withdrawal in melanoma cells. |
About Target gene
| Target gene : | MET(AUTS9,DFNB97,HGFR,RCCP2,c-Met) |
| Strong evidence: | 3C |
| Less strong evidence: | ChIP |
| Target gene experiment description: | Chromosome conformation capture (3C)was performed in COLO829 cells in the presence or absence of PLX4032 using the MET +63-kb Enhancer as an anchor region.We observed that BRAF inhibition strikingly increased the interaction between the MET +63-kb Enhancer and the MET TSS. |
About TF
| TF name : | MITF(CMM8,COMMAD,MI,WS2,WS2A,bHLHe32) |
| TF experiment: | ChIP-seq,3C |
| TF experiment description: | To test the necessity of MITF for inducible chromatin looping between the MET +63-kb Enhancer and MET TSS in response to BRAF inhibition, 3C was performed in COLO829 cells in the presence or absence of PLX4032 and MITF depletion. |
About Function
| Enhancer function : | This enhancer displays inducible chromatin looping with the MET promoter to up-regulate MET expression upon BRAF inhibition. |
| Enhancer function experiment: | ChIP,3C |
| Enhancer function experiment description: |
ChIP and 3C analysis were performed on wild-type or Enhancer-edited cells treated with PLX4032.A significant decrease in both H3K27 acetylation,a histone modification associated with active Enhancer state,and Enhancer-promoter chromatin looping was observed in Enhancer-edited cells. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| MITF | IL6-mediated signaling events | pid | 48 |
| MITF | KitReceptor | netpath | 104 |
| MITF | RANKL | netpath | 84 |
| MITF | Regulation of nuclear beta catenin signaling and target gene transcription | pid | 80 |
| MITF | Regulation of retinoblastoma protein | pid | 67 |
| MITF | Signaling events mediated by Stem cell factor receptor (c-Kit) | pid | 53 |
| MITF | Signaling mediated by p38-alpha and p38-beta | pid | 35 |
| MITF | SUMOylation of transcription factors | reactome | 14 |
| MITF | Melanogenesis | kegg | 100 |
| MITF | Pathways in cancer | kegg | 321 |
| MITF | Melanoma | kegg | 69 |
| MITF | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| MITF | Hs_Kit_receptor_signaling_pathway_WP304_78799 | wikipathways | 46 |
| MITF | Hs_RANKL-RANK_(Receptor_activator_of_NFKB_(ligand))_Signaling_Pathway_WP2018_90016 | wikipathways | 26 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene