About Enhancer
| Enhancer ID: | E_02_0910 |
| Species: | human |
| Position : | chr8:128230172-128243000   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Colorectal cancer |
| Pubmed ID: | 24594601 |
| Enhancer experiment: | qRT-PCR,3C |
| Enhancer experiment description: | The enhancer region showed long-range in_x005f_x0002_teraction with MYC (?335 kb from the region),suggesting that the enhancer region could regulate CARLo-5 (?180 kb from the region) expression by enhancing its transcription through direct interaction. To test this possibility, we performed chro_x0002_mosome conformation capture (3C) analysis using genomic DNA from CRC-derived HCT116 and RKO cells.The results of 3C analysis show that the enhancer region physically interacts with CARLo-5. |
About Target gene
| Target gene : | CCAT1(CARLO5,CARLo-5,onco-lncRNA-40) |
| Strong evidence: | 3C |
| Less strong evidence: | qRT-PCR |
| Target gene experiment description: | These results demonstrate that the enhancer region may regulate expression of CARLo-5 by direct interaction with the active regulatory 5? promoter region of CARLo-5.These results suggest that the cancer-associated variant rs6983267 in MYC enhancer could regulate CARLo-5 expression through long-range interaction with the active regulatory region of its promoter. |
About TF
| TF name : | -- |
| TF experiment: | -- |
| TF experiment description: | -- |
About Function
| Enhancer function : | The MYC Enhancer region physically interacts with the active regulatory region of the CARLo-5 promoter, suggesting long-range interaction of MYC Enhancer with the CARLo-5 promoter regulates CARLo-5 expression. |
| Enhancer function experiment: | 3C,qRT-PCR,siRNA |
| Enhancer function experiment description: |
We also found the MYC enhancer region physically interacts with the active regulatory region of the CARLo-5 promoter, suggesting long-range interaction of MYC enhancer with the CARLo-5 promoter regulates CARLo-5 expression. Finally, we demonstrate that CARLo-5 has a function in cell-cycle regulation and tumor development. |
About SNP
| SNP ID: | rs6983267 |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene