About Enhancer
| Enhancer ID: | E_02_0906 |
| Species: | human |
| Position : | chr3:184276287-184278287   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 24703906 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,qRT-PCR |
| Enhancer experiment description: | Importantly, the stimulatory capacity of the ?2.3-kb ECR differed widely among the CRC cell lines and was most pronounced in LS174T cells with highest levels of endogenous EPHB3. Thus, the EPHB3 ?2.3-kb ECR functions as a cell type-specific transcriptional enhancer. Consistent with its evolutionary conservation, also the corresponding region from the mouse EphB3 gene has enhancer properties and exhibits a remarkably similar cell-type specificity in CRC cell lines.We probed these features by chromatin immunoprecipitation (ChIP) and formaldehyde_x0002_assisted isolation of regulatory elements (FAIRE). |
About Target gene
| Target gene : | EPHB3(EK2,ETK2,HEK2,TYRO6) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,qRT-PCR |
| Target gene experiment description: | Next, we performed ChIP experiments to investigate the distri_x0002_bution of the histone modifications H3K4me1 and H3K27ac,the acetyltransferase p300, and the Wnt pathway effector TCF7L2 at the EPHB3 locus.Significantly,its cell type-specific activity matches the expression of the endogenous EPHB3 gene in CRC cell lines, suggesting that differences in enhancer function underlie differential EPHB3 expression in CRC. |
About TF
| TF name : | ASCL2(ASH2,HASH2,MASH2,bHLHa45) |
| TF experiment: | Luciferase Reporter Assay |
| TF experiment description: | we generated luciferase-reporter constructs with point mutations in the TBE and the binding sites for RBPJ and ETS factors. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene