About Enhancer
| Enhancer ID: | E_02_0902 |
| Species: | mouse |
| Position : | chr4:124638991-124641160   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 24905168 |
| Enhancer experiment: | ChIP-seq,RNA-seq |
| Enhancer experiment description: | To address whether changes in enhancer utilization patterns occur in the majority of cells within the differentiating population, we isolated dual transgenic enhancer reporter lines, in which ESC-specific or EpiLC-specific enhancers of interest were cloned upstream of a minimal promoter and one of two distinct fluorescent reporters and integrated into the ESC genome using a PiggyBac transposon system. |
About Target gene
| Target gene : | Pou3f1(Oct-6,Oct6,Otf-6,Otf6,Scip,Test1,Tst-1,Tst1) |
| Strong evidence: | -- |
| Less strong evidence: | qPCR,IP-Western |
| Target gene experiment description: | Upon differentiation, Otx2?/? cells underwent morphological changes indistinguishable from those seen in wt EpiLCs, but showed defects in expression of certain epiblast-associated genes, such as Fgf5 and Oct6. RNA-seq analysis of Otx2?/? EpiLCs identified 260 and 141 genes significantly downregulated or upregulated, respectively, as compared to the genetically-matched wt EpiLCs |
About TF
| TF name : | Otx2(E130306E05Rik)Oct4 |
| TF experiment: | ChIP-seq |
| TF experiment description: | Our results illuminate regulatory mechanisms underlying pluripotency and suggest that capacity of transcription factors such as Otx2 and Oct4 to pioneer new Enhancer sites is highly context dependent. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene