About Enhancer
| Enhancer ID: | E_02_0859 |
| Species: | human |
| Position : | chr10:64737032-64737909   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Ewing sarcoma |
| Pubmed ID: | 26214589 |
| Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay |
| Enhancer experiment description: | We observed activating chromatin marks, signals for formaldehyde-assisted isolation of regulatory elements (FAIRE) and/or DNaseI hypersensitivity at five main loci: two loci corresponding to known EGR2 regulatory elements (MSE (myelinating Schwann cell enhancer) 30 and BoneE (bone enhancer) (unpublished data); Supplementary Fig. 8), one to the ADO promoter, and two to GGAA microsatellites (mSat1 and mSat2) that overlapped with EWSR1-FLI1 ChIP-Seq signals (Fig. 3a). Luciferase reporter assays indicated that BoneE and MSE had no or weak activity in Ewing sarcoma, respectively. |
About Target gene
| Target gene : | EGR2 (AT591,CHN1,CMT1D,CMT4E,KROX20) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq,Luciferase Reporter Assay |
| Target gene experiment description: | We observed activating chromatin marks, signals for formaldehyde-assisted isolation of regulatory elements (FAIRE) and/or DNaseI hypersensitivity at five main loci: two loci corresponding to known EGR2 regulatory elements (MSE (myelinating Schwann cell enhancer) 30 and BoneE (bone enhancer) (unpublished data); Supplementary Fig. 8), one to the ADO promoter, and two to GGAA microsatellites (mSat1 and mSat2) that overlapped with EWSR1-FLI1 ChIP-Seq signals (Fig. 3a). Luciferase reporter assays indicated that BoneE and MSE had no or weak activity in Ewing sarcoma, respectively. |
About TF
| TF name : | EWSR1(EWS,EWS-FLI1,bK984G1.4) |
| TF experiment: | qRT-PCR |
| TF experiment description: | ChIP efficacy was validated by qRT-PCR using a CCND1 EWSR1-FLI1 binding site. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| EWSR1 | BARD1 signaling events | pid | 29 |
| EWSR1 | TGF_beta_Receptor | netpath | 220 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene