About Enhancer
| Enhancer ID: | E_02_0839 |
| Species: | human |
| Position : | chr12:109528035-109532509   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 26728555 |
| Enhancer experiment: | ChIP-seq,ChIP |
| Enhancer experiment description: | By performing ChIP-seq in different mESC lines and using two different antibodies, we were able to identify 973 high-confidence AFF3-binding sites.AFF3 is also de_x0002_tected in the intergenic regions within the Dlk1-Dio3 locus, which harbors a paternally methylated gDMR/ ICR (Fig. 1A). Two discrete AFF3-binding sites are located 12 kb (distal AFF3 peak) and 10 kb (proximal AFF3 peak)upstream of the promoter region of the maternally ex_x0002_pressed gene Meg3 (Fig. 1B).This AFF3 peak resides within the intergenic DMR (IG-DMR) of the Dlk1-Dio3 cluster and corresponds to the Meg3-proximal enhancer peak located just 2.4 kb from the Meg3-distal gDMR peak (Figs. 1B, 3A). While bisulfite sequencing of ChIP DNA demonstrated that AFF3 is on the inactive allele at the Meg3-distal gDMR,AFF3 is restricted to the unmethylated allele at the Meg3-proximal enhancer (Fig. 3B,C; Supplemental Fig. S4). |
About Target gene
| Target gene : | Meg3(2900016C05Rik,3110050O07Rik,6330408G06Rik,AI425946,AW108224,D12Bwg1266e,Gtl2,R74756,R75394) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq,ChIP |
| Target gene experiment description: | This AFF3 peak resides within the intergenic DMR (IG-DMR) of the Dlk1-Dio3 cluster and corresponds to the Meg3-proximal enhancer peak located just 2.4 kb from the Meg3-distal gDMR peak (Figs. 1B, 3A). While bisulfite sequencing of ChIP DNA demonstrated that AFF3 is on the inactive allele at the Meg3-distal gDMR,AFF3 is restricted to the unmethylated allele at the Meg3-proximal enhancer (Fig. 3B,C; Supplemental Fig. S4). |
About TF
| TF name : | Aff3(3222402O04Rik,A730046J16,LAF-4,Laf4) |
| TF experiment: | ChIP,ChIP-seq |
| TF experiment description: | This AFF3 peak resides within the intergenic DMR (IG-DMR) of the Dlk1-Dio3 cluster and corresponds to the Meg3-proximal enhancer peak located just 2.4 kb from the Meg3-distal gDMR peak (Figs. 1B, 3A). While bisulfite sequencing of ChIP DNA demonstrated that AFF3 is on the inactive allele at the Meg3-distal gDMR,AFF3 is restricted to the unmethylated allele at the Meg3-proximal enhancer (Fig. 3B,C; Supplemental Fig. S4).Knockdown of AFF3 leads to reduced H3K27ac at the Meg3-proximal enhancer, indicating that AFF3 is required to maintain its active chromatin state (Fig. 3D; Supple_x0002_mental Table 2). Furthermore, the expression levels of Meg3 and the downstream maternally expressed noncod_x0002_ing genes within the Dlk1-Dio3 locus are also reduced in AFF3 knockdown ESCs (Fig. 3E; Supplemental Fig. S5A),suggesting that an activating function of AFF3 might beexerted from the nearby Meg3-proximal enhancer. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene