About Enhancer
| Enhancer ID: | E_02_0753 |
| Species: | human |
| Position : | chr14:76419740-76421740   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Prostate cancer |
| Pubmed ID: | 30511964 |
| Enhancer experiment: | ChIP-seq,ChIP-qPCR |
| Enhancer experiment description: | Analysis of FOXA1 ChIP-seq previously performed in LNCaP cells identified strong FOXA1 binding at an enhancer of approximately 3.7 kb upstream of the TGFB3 gene promoter. |
About Target gene
| Target gene : | TGFB3(ARVD,ARVD1,LDS5,RNHF,TGF-beta3) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-qPCR |
| Target gene experiment description: | Analysis of FOXA1 ChIP-seq previously performed in LNCaP cells identified strong FOXA1 binding at an Enhancer of approximately 3.7 kb upstream of the TGFB3 gene promoter.Importantly, qRT-PCR analysis demonstrated that CRISPR-mediated Enhancer deletion in only one-fourth of the cell population was able to substantially restore the transcription of TGFB3 gene, comparing cells transfected with TGFB3-targeting gRNAs to those with control gRNAs. |
About TF
| TF name : | FOXA1(HNF3A,TCF3A) |
| TF experiment: | CRISPR/Cas9 |
| TF experiment description: | To demonstrate that FOXA1 binding at the upstream Enhancer indeed inhibits TGFB3 gene transcription in vivo, we used a lentiviral CRISPR/Cas9 editing system to delete the Enhancer elements with small guide RNAs.Taken together, these results strongly support that FOXA1 binds to an upstream Enhancer to directly repress TGFB3 transcription. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| FOXA1 | AndrogenReceptor | netpath | 167 |
| FOXA1 | Direct p53 effectors | pid | 141 |
| FOXA1 | FOXA1 transcription factor network | pid | 45 |
| FOXA1 | FOXA2 and FOXA3 transcription factor networks | pid | 45 |
| FOXA1 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene