About Enhancer
| Enhancer ID: | E_02_0661 |
| Species: | human |
| Position : | chr22:23067631-23069631   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Cancer (cancer) |
| Pubmed ID: | 31235524 |
| Enhancer experiment: | PCR, Western blot, gel electrophoresis |
| Enhancer experiment description: | Briefly, the GNAZ gene in previously established HEK293 cells devoid of three G? families (the G?s, the G?q, and the G?12 families) was mutated by a CRISPR/ Cas9 system using a GNAZ sgRNA-targeting sequence (5-GATGCGGGTCAGCGAGTCGATGG-3; including a NGG PAM sequence). Introduction of a 5-bp deletion (frame-shift mutation) into the GNAZ gene was verified by direct sequencing as described elsewhere. |
About Target gene
| Target gene : | GNAZ |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Briefly, the GNAZ gene in previously established HEK293 cells devoid of three G? families (the G?s, the G?q, and the G?12 families) was mutated by a CRISPR/ Cas9 system using a GNAZ sgRNA-targeting sequence (5-GATGCGGGTCAGCGAGTCGATGG-3; including a NGG PAM sequence). Introduction of a 5-bp deletion (frame-shift mutation) into the GNAZ gene was verified by direct sequencing as described elsewhere. |
About TF
| TF name : | -- |
| TF experiment: | PCR,Western blot,???? |
| TF experiment description: | Briefly, the GNAZ gene in previously established HEK293 cells devoid of three G? families (the G?s, the G?q, and the G?12 families) was mutated by a CRISPR/ Cas9 system using a GNAZ sgRNA-targeting sequence (5-GATGCGGGTCAGCGAGTCGATGG-3; including a NGG PAM sequence). Introduction of a 5-bp deletion (frame-shift mutation) into the GNAZ gene was verified by direct sequencing as described elsewhere. |
About Function
| Enhancer function : | Briefly, the GNAZ gene in previously established HEK293 cells devoid of three G? families (the G?s, the G?q, and the G?12 families) was mutated by a CRISPR/ Cas9 system using a GNAZ sgRNA-targeting sequence (5-GATGCGGGTCAGCGAGTCGATGG-3; including a NGG PAM sequence). Introduction of a 5-bp deletion (frame-shift mutation) into the GNAZ gene was verified by direct sequencing as described elsewhere. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Briefly, the GNAZ gene in previously established HEK293 cells devoid of three G? families (the G?s, the G?q, and the G?12 families) was mutated by a CRISPR/ Cas9 system using a GNAZ sgRNA-targeting sequence (5-GATGCGGGTCAGCGAGTCGATGG-3; including a NGG PAM sequence). Introduction of a 5-bp deletion (frame-shift mutation) into the GNAZ gene was verified by direct sequencing as described elsewhere. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene