About Enhancer
| Enhancer ID: | E_02_0595 |
| Species: | human |
| Position : | chr19:33792836-33794836   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 33137828 |
| Enhancer experiment: | Cell transfection, dual luciferase reporter assay, RNA extraction and qPCR analysis |
| Enhancer experiment description: | We identified miR-381, which specifically targets the 3? untranslated region (3? UTR) of potassium channel tetramerization-domain-containing 15 (KCTD15) , and verified the mechanism regulating its expression and participation in adipogenesis. We used a dual luciferase-reporter assay and transfection-mediated miR-381 overexpression and inhibition in Yanbian yellow cattle preadipocytes to investigate the role of miR-381 in adipogenesis. |
About Target gene
| Target gene : | KCTD15 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | We identified miR-381, which specifically targets the 3? untranslated region (3? UTR) of potassium channel tetramerization-domain-containing 15 (KCTD15) , and verified the mechanism regulating its expression and participation in adipogenesis. We used a dual luciferase-reporter assay and transfection-mediated miR-381 overexpression and inhibition in Yanbian yellow cattle preadipocytes to investigate the role of miR-381 in adipogenesis. |
About TF
| TF name : | -- |
| TF experiment: | ?????Dual luciferase-reporter assay?RNA extraction and qPCR analysis |
| TF experiment description: | We identified miR-381, which specifically targets the 3? untranslated region (3? UTR) of potassium channel tetramerization-domain-containing 15 (KCTD15) , and verified the mechanism regulating its expression and participation in adipogenesis. We used a dual luciferase-reporter assay and transfection-mediated miR-381 overexpression and inhibition in Yanbian yellow cattle preadipocytes to investigate the role of miR-381 in adipogenesis. |
About Function
| Enhancer function : | We identified miR-381, which specifically targets the 3? untranslated region (3? UTR) of potassium channel tetramerization-domain-containing 15 (KCTD15) , and verified the mechanism regulating its expression and participation in adipogenesis. We used a dual luciferase-reporter assay and transfection-mediated miR-381 overexpression and inhibition in Yanbian yellow cattle preadipocytes to investigate the role of miR-381 in adipogenesis. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
We identified miR-381, which specifically targets the 3? untranslated region (3? UTR) of potassium channel tetramerization-domain-containing 15 (KCTD15) , and verified the mechanism regulating its expression and participation in adipogenesis. We used a dual luciferase-reporter assay and transfection-mediated miR-381 overexpression and inhibition in Yanbian yellow cattle preadipocytes to investigate the role of miR-381 in adipogenesis. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene