About Enhancer
| Enhancer ID: | E_02_0581 |
| Species: | human |
| Position : | chr3:187718918-187720918   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | B cell lymphoma |
| Pubmed ID: | 33232656 |
| Enhancer experiment: | Cross linked chromatin immunoprecipitation (chip), gene knockdown, CRISPR / cas9, RNA isolation, cDNA preparation and quantitative real-time PCR (qPCR), luciferase assay, immunoprecipitation and immunoprecipitation mass spectrometry (ip-ms), electrophoretic mobility shift assay (EMSA |
| Enhancer experiment description: | Here we show that OCA-B, a B cell-specific coactivator essential for germinal center (GC) formation, forms a ternary complex with the lymphoid-enriched OCT2 and GC-specific MEF2B transcription factors, and that this complex occupies and activates an LCR that regulates the BCL6 proto-oncogene and is uniquely required by normal and malignant GC B-cells. |
About Target gene
| Target gene : | BCL6(BCL5A,LAZ3,ZBTB27,ZNF51,BCL6) |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Here we show that OCA-B, a B cell-specific coactivator essential for germinal center (GC) formation, forms a ternary complex with the lymphoid-enriched OCT2 and GC-specific MEF2B transcription factors, and that this complex occupies and activates an LCR that regulates the BCL6 proto-oncogene and is uniquely required by normal and malignant GC B-cells. |
About TF
| TF name : | -- |
| TF experiment: | ????????? ?ChIP???????CRISPR/Cas9?RNA ???cDNA ??????????? PCR ?qPCR?????????????????????IP-MS??????????? ?EMSA? |
| TF experiment description: | Here we show that OCA-B, a B cell-specific coactivator essential for germinal center (GC) formation, forms a ternary complex with the lymphoid-enriched OCT2 and GC-specific MEF2B transcription factors, and that this complex occupies and activates an LCR that regulates the BCL6 proto-oncogene and is uniquely required by normal and malignant GC B-cells. |
About Function
| Enhancer function : | Here we show that OCA-B, a B cell-specific coactivator essential for germinal center (GC) formation, forms a ternary complex with the lymphoid-enriched OCT2 and GC-specific MEF2B transcription factors, and that this complex occupies and activates an LCR that regulates the BCL6 proto-oncogene and is uniquely required by normal and malignant GC B-cells. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Here we show that OCA-B, a B cell-specific coactivator essential for germinal center (GC) formation, forms a ternary complex with the lymphoid-enriched OCT2 and GC-specific MEF2B transcription factors, and that this complex occupies and activates an LCR that regulates the BCL6 proto-oncogene and is uniquely required by normal and malignant GC B-cells. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene