About Enhancer
| Enhancer ID: | E_02_0560 |
| Species: | human |
| Position : | chr4:108044986-108046986   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Cystic kidney disease |
| Pubmed ID: | 33453998 |
| Enhancer experiment: | Gene knockdown, luciferase reporter assay, genome wide chip SEQ analysis |
| Enhancer experiment description: | We show that ablation of HNF-1? in mIMCD3 renal epithelial cells results in activation of ?-catenin and increased expression of lymphoid enhancerbinding factor 1 (LEF1), a downstream effector in the canonical Wnt signaling pathway. Increased expression and nuclear localization of LEF1 are also observed in cystic kidneys from Hnf1b mutant mice. |
About Target gene
| Target gene : | LEF1 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | We show that ablation of HNF-1? in mIMCD3 renal epithelial cells results in activation of ?-catenin and increased expression of lymphoid enhancerbinding factor 1 (LEF1), a downstream effector in the canonical Wnt signaling pathway. Increased expression and nuclear localization of LEF1 are also observed in cystic kidneys from Hnf1b mutant mice. |
About TF
| TF name : | -- |
| TF experiment: | ????,?????????????ChIP-Seq?? |
| TF experiment description: | We show that ablation of HNF-1? in mIMCD3 renal epithelial cells results in activation of ?-catenin and increased expression of lymphoid enhancerbinding factor 1 (LEF1), a downstream effector in the canonical Wnt signaling pathway. Increased expression and nuclear localization of LEF1 are also observed in cystic kidneys from Hnf1b mutant mice. |
About Function
| Enhancer function : | We show that ablation of HNF-1? in mIMCD3 renal epithelial cells results in activation of ?-catenin and increased expression of lymphoid enhancerbinding factor 1 (LEF1), a downstream effector in the canonical Wnt signaling pathway. Increased expression and nuclear localization of LEF1 are also observed in cystic kidneys from Hnf1b mutant mice. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
We show that ablation of HNF-1? in mIMCD3 renal epithelial cells results in activation of ?-catenin and increased expression of lymphoid enhancerbinding factor 1 (LEF1), a downstream effector in the canonical Wnt signaling pathway. Increased expression and nuclear localization of LEF1 are also observed in cystic kidneys from Hnf1b mutant mice. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene