About Enhancer
| Enhancer ID: | E_02_0538 |
| Species: | human |
| Position : | chr1:116751815-116753815   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 29985393 |
| Enhancer experiment: | RNA SEQ, RNA SEQ, statistical analysis, flow cytometry |
| Enhancer experiment description: | Go to: Results Characteristics of thymic iNKT cell subsets We analyzed thymic iNKT cells from BALB/c mice in which the human CD2 gene was knocked-in into the IL-4 locus (KN2), thereby allowing for the identification by flow cytometry of IL-4-secreting iNKT2 cells directly ex vivo. Development of iNKT cells diverges from that of conventional T cells primarily at the double-positive CD4+ CD8+ (DP) stage and requires TCR recognition of CD1d on DP cells, involving homotypic interactions across a DP DP synapse where second signals are initiated by the engagement of homophilic receptors of the signaling lymphocytic-activation molecule (SLAM) family, Slamf1 (SLAM) and Slamf6 (Ly108). |
About Target gene
| Target gene : | CD2 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Go to: Results Characteristics of thymic iNKT cell subsets We analyzed thymic iNKT cells from BALB/c mice in which the human CD2 gene was knocked-in into the IL-4 locus (KN2), thereby allowing for the identification by flow cytometry of IL-4-secreting iNKT2 cells directly ex vivo. Development of iNKT cells diverges from that of conventional T cells primarily at the double-positive CD4+ CD8+ (DP) stage and requires TCR recognition of CD1d on DP cells, involving homotypic interactions across a DP DP synapse where second signals are initiated by the engagement of homophilic receptors of the signaling lymphocytic-activation molecule (SLAM) family, Slamf1 (SLAM) and Slamf6 (Ly108). |
About TF
| TF name : | -- |
| TF experiment: | RNA-Seq,RNA-Seq,????,????? |
| TF experiment description: | Go to: Results Characteristics of thymic iNKT cell subsets We analyzed thymic iNKT cells from BALB/c mice in which the human CD2 gene was knocked-in into the IL-4 locus (KN2), thereby allowing for the identification by flow cytometry of IL-4-secreting iNKT2 cells directly ex vivo. Development of iNKT cells diverges from that of conventional T cells primarily at the double-positive CD4+ CD8+ (DP) stage and requires TCR recognition of CD1d on DP cells, involving homotypic interactions across a DP DP synapse where second signals are initiated by the engagement of homophilic receptors of the signaling lymphocytic-activation molecule (SLAM) family, Slamf1 (SLAM) and Slamf6 (Ly108). |
About Function
| Enhancer function : | Go to: Results Characteristics of thymic iNKT cell subsets We analyzed thymic iNKT cells from BALB/c mice in which the human CD2 gene was knocked-in into the IL-4 locus (KN2), thereby allowing for the identification by flow cytometry of IL-4-secreting iNKT2 cells directly ex vivo. Development of iNKT cells diverges from that of conventional T cells primarily at the double-positive CD4+ CD8+ (DP) stage and requires TCR recognition of CD1d on DP cells, involving homotypic interactions across a DP DP synapse where second signals are initiated by the engagement of homophilic receptors of the signaling lymphocytic-activation molecule (SLAM) family, Slamf1 (SLAM) and Slamf6 (Ly108). |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Go to: Results Characteristics of thymic iNKT cell subsets We analyzed thymic iNKT cells from BALB/c mice in which the human CD2 gene was knocked-in into the IL-4 locus (KN2), thereby allowing for the identification by flow cytometry of IL-4-secreting iNKT2 cells directly ex vivo. Development of iNKT cells diverges from that of conventional T cells primarily at the double-positive CD4+ CD8+ (DP) stage and requires TCR recognition of CD1d on DP cells, involving homotypic interactions across a DP DP synapse where second signals are initiated by the engagement of homophilic receptors of the signaling lymphocytic-activation molecule (SLAM) family, Slamf1 (SLAM) and Slamf6 (Ly108). |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene