About Enhancer
| Enhancer ID: | E_02_0506 |
| Species: | human |
| Position : | chr10:96186235-96188235   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 30055251 |
| Enhancer experiment: | Western blot, immunofluorescence staining |
| Enhancer experiment description: | Functional studies involving BLNK were first car- ried out in chicken BLNK-deficient DT40 cells, which exhibited the abolishment of phospholipase C-?2 (PLC-?2) tyrosine phosphorylation, a calcium mobilization defect, and impaired activation of c-Jun N- terminal kinase (JNK) [4]. After SYK phosphorylates several critical tyrosines (Y72, Y84, Y96, Y178, and Y189), BLNK provides docking sites for intracellular sig- naling molecules including guanine nucleotide exchange factors (VAV), non-catalytic region of tyrosine kinase (NCK), Bruton's tyrosine kinase (BTK), and phospholipase C? (PLC?) [1 3]. |
About Target gene
| Target gene : | BLNK |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Functional studies involving BLNK were first car- ried out in chicken BLNK-deficient DT40 cells, which exhibited the abolishment of phospholipase C-?2 (PLC-?2) tyrosine phosphorylation, a calcium mobilization defect, and impaired activation of c-Jun N- terminal kinase (JNK) [4]. After SYK phosphorylates several critical tyrosines (Y72, Y84, Y96, Y178, and Y189), BLNK provides docking sites for intracellular sig- naling molecules including guanine nucleotide exchange factors (VAV), non-catalytic region of tyrosine kinase (NCK), Bruton's tyrosine kinase (BTK), and phospholipase C? (PLC?) [1 3]. |
About TF
| TF name : | -- |
| TF experiment: | Western blot,?????? |
| TF experiment description: | Functional studies involving BLNK were first car- ried out in chicken BLNK-deficient DT40 cells, which exhibited the abolishment of phospholipase C-?2 (PLC-?2) tyrosine phosphorylation, a calcium mobilization defect, and impaired activation of c-Jun N- terminal kinase (JNK) [4]. After SYK phosphorylates several critical tyrosines (Y72, Y84, Y96, Y178, and Y189), BLNK provides docking sites for intracellular sig- naling molecules including guanine nucleotide exchange factors (VAV), non-catalytic region of tyrosine kinase (NCK), Bruton's tyrosine kinase (BTK), and phospholipase C? (PLC?) [1 3]. |
About Function
| Enhancer function : | Functional studies involving BLNK were first car- ried out in chicken BLNK-deficient DT40 cells, which exhibited the abolishment of phospholipase C-?2 (PLC-?2) tyrosine phosphorylation, a calcium mobilization defect, and impaired activation of c-Jun N- terminal kinase (JNK) [4]. After SYK phosphorylates several critical tyrosines (Y72, Y84, Y96, Y178, and Y189), BLNK provides docking sites for intracellular sig- naling molecules including guanine nucleotide exchange factors (VAV), non-catalytic region of tyrosine kinase (NCK), Bruton's tyrosine kinase (BTK), and phospholipase C? (PLC?) [1 3]. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Functional studies involving BLNK were first car- ried out in chicken BLNK-deficient DT40 cells, which exhibited the abolishment of phospholipase C-?2 (PLC-?2) tyrosine phosphorylation, a calcium mobilization defect, and impaired activation of c-Jun N- terminal kinase (JNK) [4]. After SYK phosphorylates several critical tyrosines (Y72, Y84, Y96, Y178, and Y189), BLNK provides docking sites for intracellular sig- naling molecules including guanine nucleotide exchange factors (VAV), non-catalytic region of tyrosine kinase (NCK), Bruton's tyrosine kinase (BTK), and phospholipase C? (PLC?) [1 3]. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene