About Enhancer
| Enhancer ID: | E_02_0458 |
| Species: | mouse |
| Position : | chr15:76897064-76899064   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 30146317 |
| Enhancer experiment: | QRT PCR, chipseq, statistical analysis, immunofluorescence staining |
| Enhancer experiment description: | Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). |
About Target gene
| Target gene : | Mb,Lsm8,Akr1b8,Shh(HHG1,HLP3,HPE3,MCOPCB5,SMMCI,ShhNC,TPT,TPTPS) |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). ;Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). ;Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). ;Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). |
About TF
| TF name : | -- |
| TF experiment: | qRT-PCR, ChIPseq,????,?????? |
| TF experiment description: | Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). ;Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). ;Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). ;Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). |
About Function
| Enhancer function : | Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Gene regulation requires selective targeting of DNA regulatory enhancers over megabase (Mb) distances. Transgene expression of Evf2 activates Lsm8 (12Mb distant), but fails to repress Akr1b8, supporting trans-activation and long-range cis repression. Early studies showed regulatory interactions between the sonic hedgehog (Shh) limb Enh (ZRS) and the Shh gene, despite 1Mb distance (Lettice et al., 2003). |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene