About Enhancer
| Enhancer ID: | E_02_0404 |
| Species: | human |
| Position : | chr16:10440279-10442279   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 30327417 |
| Enhancer experiment: | qChIP |
| Enhancer experiment description: | We then analyzed peak A and Ciita promoter I by qChIP in NFAT5-deficient or control macrophages for different histone modifications that are enriched in gene enhancers (H3K4me1) or promoters (H3K4me3) and a modification that marks transcribed regions and active enhancers.We observed that peak A region had a slightly higher content in H3K4me1 compared with Ciita promoter I, and this was NFAT5 independent. |
About Target gene
| Target gene : | Ciita(C2ta,EG669998,Gm9475,Mhc2ta) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq |
| Target gene experiment description: | ChIP-seq analysis of wild-type and NFAT5-deficient BMDMs showing the position of NFAT5-binding site peak A 47 kb upstream of the Ciita locus. |
About TF
| TF name : | Nfat5(AI225870,B130038B15Rik,CAG-8,CAG80,NFATL1,OREBP,TonEBP,mKIAA0827,nfatz) |
| TF experiment: | qChIP |
| TF experiment description: | We therefore asked whether NFAT5 bound these regulatory regions. Quantitative chromatin immunoprecipitation (qChIP) with a combination of two polyclonal antibodies to NFAT5 detected its specific binding to promoter I of Ciita and the promoter of H2-Ab both in untreated macrophages as well as in macrophages treated with IFN?.This result indicated that NFAT5 is a relevant factor to control Ciita expression in macrophages through its myeloid promoter I. Altogether,our analysis of primary myeloid and lymphoid APCs uncovered a selective requirement of NFAT5 in macrophages for expressing CIITA and MHCII. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Nfat5 | Inflammation mediated by chemokine and cytokine signaling pathway | panther | 189 |
| Nfat5 | Wnt signaling pathway | kegg | 135 |
| Nfat5 | Axon guidance | kegg | 126 |
| Nfat5 | VEGF signaling pathway | kegg | 70 |
| Nfat5 | Natural killer cell mediated cytotoxicity | kegg | 125 |
| Nfat5 | T cell receptor signaling pathway | kegg | 105 |
| Nfat5 | B cell receptor signaling pathway | kegg | 70 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene