About Enhancer
| Enhancer ID: |
E_02_0384 |
| Species: |
mouse |
| Position :
|
chr1:78074974-78076974
  |
| Biosample name: |
|
| Experiment class :
|
High+Lowthroughput |
| Enhancer type: |
Enhancer |
| Disease: |
-- |
| Pubmed ID: |
30453072
|
| Enhancer experiment: |
Immunohistochemical analysis?Real-time PCR |
| Enhancer experiment description: |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
About Target gene
| Target gene : |
Pax3(CDHS,HUP2,WS1,WS3) |
| Strong evidence: |
qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: |
RNA-Seq |
| Target gene experiment description: |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
About TF
| TF name : |
-- |
| TF experiment: |
Immunohistochemical analysis?Real-time PCR |
| TF experiment description: |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
About Function
| Enhancer function : |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
| Enhancer function experiment: |
Immunohistochemical staining |
Enhancer function
experiment description: |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
About SNP
Upstream Pathway Annotation of TF
| GeneName |
Pathway Name |
Source |
Gene Number |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene
Expression of target genes for the
enhancer
Enhancer associated SNPs