About Enhancer
| Enhancer ID: | E_02_0381 |
| Species: | human |
| Position : | chr8:144312388-144314388   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Hepatic steatosis, obesity |
| Pubmed ID: | 30463291 |
| Enhancer experiment: | Cell Viability Assay,Western Blot Analysis,Oil Red O Staining |
| Enhancer experiment description: | The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver. |
About Target gene
| Target gene : | DGAT1,FAS,CPT2 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver.;The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver.;The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver. |
About TF
| TF name : | -- |
| TF experiment: | Cell Viability Assay,Western Blot Analysis,Oil Red O Staining |
| TF experiment description: | The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver.;The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver.;The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver. |
About Function
| Enhancer function : | The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBP? and PPAR?, increases in expression of lipolytic enzymes, such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in WAT of HFD-fed mice. In addition, IOE-treated mice had lower hepatic TG content, associated with lower protein expression of lipogenic genes, such as diglyceride acyltransferase 1 (DGAT1), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). IOE treatment also reduced serum free fatty acid concentration, probably through the upregulation of ?-oxidation genes, suggested by increases in AMPK? and CPT1 expression in WAT and liver. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene