About Enhancer
| Enhancer ID: | E_02_0354 |
| Species: | human |
| Position : | chr6:45325492-45327492   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Osteogenic differentiation |
| Pubmed ID: | 32324256 |
| Enhancer experiment: | ChIP and Re?ChIP assays?RNA extraction?quantitative reverse transcription PCR?RNA extraction?quantitative reverse transcription PCR |
| Enhancer experiment description: | RUNX2 and EGR1 co repressed Htra1 and increased the expression levels of other osteoblast marker genes, such as osterix, osteocalcin, and osteoprotegerin at the messenger RNA and protein level. |
About Target gene
| Target gene : | RUNX2(AML3,CBF-alpha-1,CBFA1,CCD,CCD1,CLCD,OSF-2,OSF2,PEA2aA,PEBP2aA),EGR1 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | RUNX2 and EGR1 co repressed Htra1 and increased the expression levels of other osteoblast marker genes, such as osterix, osteocalcin, and osteoprotegerin at the messenger RNA and protein level.;RUNX2 and EGR1 co repressed Htra1 and increased the expression levels of other osteoblast marker genes, such as osterix, osteocalcin, and osteoprotegerin at the messenger RNA and protein level. |
About TF
| TF name : | -- |
| TF experiment: | ChIP and Re?ChIP assays?RNA extraction?quantitative reverse transcription PCR?RNA extraction?quantitative reverse transcription PCR |
| TF experiment description: | RUNX2 and EGR1 co repressed Htra1 and increased the expression levels of other osteoblast marker genes, such as osterix, osteocalcin, and osteoprotegerin at the messenger RNA and protein level.;RUNX2 and EGR1 co repressed Htra1 and increased the expression levels of other osteoblast marker genes, such as osterix, osteocalcin, and osteoprotegerin at the messenger RNA and protein level. |
About Function
| Enhancer function : | RUNX2 and EGR1 co repressed Htra1 and increased the expression levels of other osteoblast marker genes, such as osterix, osteocalcin, and osteoprotegerin at the messenger RNA and protein level. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
RUNX2 and EGR1 co repressed Htra1 and increased the expression levels of other osteoblast marker genes, such as osterix, osteocalcin, and osteoprotegerin at the messenger RNA and protein level. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene