About Enhancer
| Enhancer ID: | E_02_0316 |
| Species: | human |
| Position : | chr18:59217032-59219032   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Spinal cord injury (sci) |
| Pubmed ID: | 29731818 |
| Enhancer experiment: | Basso, Beattie and Bresnahan (BBB) scoring,MTT colorimetric assay,Western blot,Apoptosis assay,Terminal deoxynucleotidyl?transferase?mediated dUTP nick end (TUNEL),ELISA,flow cytometry, |
| Enhancer experiment description: | Furthermore, levels of ERS-associated proteins, including caspase-3, activating transcription factor 6, serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 ?, eukaryotic initiation factor 2 ? and GRP78, were significantly increased following SCI; however, administration of MTX for 7 days significantly reversed this effect (P<0.05, P<0.01 and P<0.001). Therefore, MTX may improve SCI by suppressing ERS-induced apoptosis in vitro and in vivo. |
About Target gene
| Target gene : | GRP |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Furthermore, levels of ERS-associated proteins, including caspase-3, activating transcription factor 6, serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 ?, eukaryotic initiation factor 2 ? and GRP78, were significantly increased following SCI; however, administration of MTX for 7 days significantly reversed this effect (P<0.05, P<0.01 and P<0.001). Therefore, MTX may improve SCI by suppressing ERS-induced apoptosis in vitro and in vivo. |
About TF
| TF name : | -- |
| TF experiment: | Basso, Beattie and Bresnahan (BBB) scoring,MTT colorimetric assay,Western blot,Apoptosis assay,Terminal deoxynucleotidyl?transferase?mediated dUTP nick end (TUNEL),ELISA,flow cytometry, |
| TF experiment description: | Furthermore, levels of ERS-associated proteins, including caspase-3, activating transcription factor 6, serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 ?, eukaryotic initiation factor 2 ? and GRP78, were significantly increased following SCI; however, administration of MTX for 7 days significantly reversed this effect (P<0.05, P<0.01 and P<0.001). Therefore, MTX may improve SCI by suppressing ERS-induced apoptosis in vitro and in vivo. |
About Function
| Enhancer function : | Furthermore, levels of ERS-associated proteins, including caspase-3, activating transcription factor 6, serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 ?, eukaryotic initiation factor 2 ? and GRP78, were significantly increased following SCI; however, administration of MTX for 7 days significantly reversed this effect (P<0.05, P<0.01 and P<0.001). Therefore, MTX may improve SCI by suppressing ERS-induced apoptosis in vitro and in vivo. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Furthermore, levels of ERS-associated proteins, including caspase-3, activating transcription factor 6, serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 ?, eukaryotic initiation factor 2 ? and GRP78, were significantly increased following SCI; however, administration of MTX for 7 days significantly reversed this effect (P<0.05, P<0.01 and P<0.001). Therefore, MTX may improve SCI by suppressing ERS-induced apoptosis in vitro and in vivo. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene