About Enhancer
| Enhancer ID: | E_02_0255 |
| Species: | human |
| Position : | chr17:42310249-42312249   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Leukemia |
| Pubmed ID: | 30630179 |
| Enhancer experiment: | Immunohistochemistry, cell transfection, PCR, Western blot |
| Enhancer experiment description: | The expression vectors for wild type (WT) STAT3 (Stat3 Flag pRC/CMV, #8707, Addgene, Cambridge), dominant negative (DN) STAT3 (Stat3 Y705F Flag pRC/CMV, #8704, Addgene, Cambridge), constitutively active (CA) STAT3 (Stat3-C Flag pRC/CMV, #8722, Addgene, Cambridge), and luciferase reporter containing STAT3 response element (SBR, 4xM67 pTATA TK-Luc, #8688, Addgene, Cambridge, MA) were purchased from Addgene and have previously been described [74]. |
About Target gene
| Target gene : | STAT3(1110034C02Rik,AW109958,Aprf) |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | The expression vectors for wild type (WT) STAT3 (Stat3 Flag pRC/CMV, #8707, Addgene, Cambridge), dominant negative (DN) STAT3 (Stat3 Y705F Flag pRC/CMV, #8704, Addgene, Cambridge), constitutively active (CA) STAT3 (Stat3-C Flag pRC/CMV, #8722, Addgene, Cambridge), and luciferase reporter containing STAT3 response element (SBR, 4xM67 pTATA TK-Luc, #8688, Addgene, Cambridge, MA) were purchased from Addgene and have previously been described [74]. |
About TF
| TF name : | -- |
| TF experiment: | ??????????PCR?Western blot |
| TF experiment description: | The expression vectors for wild type (WT) STAT3 (Stat3 Flag pRC/CMV, #8707, Addgene, Cambridge), dominant negative (DN) STAT3 (Stat3 Y705F Flag pRC/CMV, #8704, Addgene, Cambridge), constitutively active (CA) STAT3 (Stat3-C Flag pRC/CMV, #8722, Addgene, Cambridge), and luciferase reporter containing STAT3 response element (SBR, 4xM67 pTATA TK-Luc, #8688, Addgene, Cambridge, MA) were purchased from Addgene and have previously been described [74]. |
About Function
| Enhancer function : | The expression vectors for wild type (WT) STAT3 (Stat3 Flag pRC/CMV, #8707, Addgene, Cambridge), dominant negative (DN) STAT3 (Stat3 Y705F Flag pRC/CMV, #8704, Addgene, Cambridge), constitutively active (CA) STAT3 (Stat3-C Flag pRC/CMV, #8722, Addgene, Cambridge), and luciferase reporter containing STAT3 response element (SBR, 4xM67 pTATA TK-Luc, #8688, Addgene, Cambridge, MA) were purchased from Addgene and have previously been described [74]. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
The expression vectors for wild type (WT) STAT3 (Stat3 Flag pRC/CMV, #8707, Addgene, Cambridge), dominant negative (DN) STAT3 (Stat3 Y705F Flag pRC/CMV, #8704, Addgene, Cambridge), constitutively active (CA) STAT3 (Stat3-C Flag pRC/CMV, #8722, Addgene, Cambridge), and luciferase reporter containing STAT3 response element (SBR, 4xM67 pTATA TK-Luc, #8688, Addgene, Cambridge, MA) were purchased from Addgene and have previously been described [74]. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene