About Enhancer
| Enhancer ID: | E_02_0189 |
| Species: | human |
| Position : | chr7:148804549-148806549   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Infectious gastroenteritis |
| Pubmed ID: | 31592229 |
| Enhancer experiment: | Western blot, flow cytometry, PCR, immunofluorescence staining |
| Enhancer experiment description: | In our previous study, we have demonstrated that circular RNA circEZH2 was down-regulated during TGEV infection and promoted the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) via targeting miR-22 in porcine intestinal epithelial cell line (IPEC-J2). |
About Target gene
| Target gene : | EZH2,KAT6B,HK2 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | In our previous study, we have demonstrated that circular RNA circEZH2 was down-regulated during TGEV infection and promoted the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) via targeting miR-22 in porcine intestinal epithelial cell line (IPEC-J2).;miR-22 has a strong correlation with NF-?B pathway and mitochondrial damage. Recently, it was reported that miR-22 inhibited PI3K/Akt/NF-?B signaling in tongue squamous cell carcinoma (TSCC) cells via targeting KAT6B[22].;However, HK2 does not regulate NK-?B activation. Not only HK2, IL-6 was also identified as the target of miR-22 and suppressed TGEV-induced mPTP opening via activating NF-?B pathway, suggesting that miR-22 regulates TGEV-induced mPTP opening via at least two pathways. |
About TF
| TF name : | -- |
| TF experiment: | Western blot,Flow cytometry,PCR,?????? |
| TF experiment description: | In our previous study, we have demonstrated that circular RNA circEZH2 was down-regulated during TGEV infection and promoted the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) via targeting miR-22 in porcine intestinal epithelial cell line (IPEC-J2).;miR-22 has a strong correlation with NF-?B pathway and mitochondrial damage. Recently, it was reported that miR-22 inhibited PI3K/Akt/NF-?B signaling in tongue squamous cell carcinoma (TSCC) cells via targeting KAT6B[22].;However, HK2 does not regulate NK-?B activation. Not only HK2, IL-6 was also identified as the target of miR-22 and suppressed TGEV-induced mPTP opening via activating NF-?B pathway, suggesting that miR-22 regulates TGEV-induced mPTP opening via at least two pathways. |
About Function
| Enhancer function : | In our previous study, we have demonstrated that circular RNA circEZH2 was down-regulated during TGEV infection and promoted the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) via targeting miR-22 in porcine intestinal epithelial cell line (IPEC-J2). |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
In our previous study, we have demonstrated that circular RNA circEZH2 was down-regulated during TGEV infection and promoted the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) via targeting miR-22 in porcine intestinal epithelial cell line (IPEC-J2). |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene