About Enhancer
| Enhancer ID: | E_02_0150 |
| Species: | human |
| Position : | chr1:156461181-156463181   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Liver cancer |
| Pubmed ID: | 31678303 |
| Enhancer experiment: | Western blot, PCR, flow cytometry, immunofluorescence staining |
| Enhancer experiment description: | MEF2D protein acetylation and proteins that interact with MEF2D were identified by co-immunoprecipitation and pull-down assays. H22 cells, with MEF2D knockout or without (controls) were transplanted into BALB/c mice, some mice were given antibodies to deplete T cells. |
About Target gene
| Target gene : | MEF2D,SIRT7,CD274 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | MEF2D protein acetylation and proteins that interact with MEF2D were identified by co-immunoprecipitation and pull-down assays. H22 cells, with MEF2D knockout or without (controls) were transplanted into BALB/c mice, some mice were given antibodies to deplete T cells. ;Mice bearing orthotopic tumors grown from HCC cells with or without knockout of SIRT7, were given injections of an antibody against PD1.;MEF2D bound the promoter region of the CD274 gene (encodes PD-L1) and activated its transcription. |
About TF
| TF name : | -- |
| TF experiment: | Western blot,PCR,Flow cytometry,?????? |
| TF experiment description: | MEF2D protein acetylation and proteins that interact with MEF2D were identified by co-immunoprecipitation and pull-down assays. H22 cells, with MEF2D knockout or without (controls) were transplanted into BALB/c mice, some mice were given antibodies to deplete T cells. ;Mice bearing orthotopic tumors grown from HCC cells with or without knockout of SIRT7, were given injections of an antibody against PD1.;MEF2D bound the promoter region of the CD274 gene (encodes PD-L1) and activated its transcription. |
About Function
| Enhancer function : | MEF2D protein acetylation and proteins that interact with MEF2D were identified by co-immunoprecipitation and pull-down assays. H22 cells, with MEF2D knockout or without (controls) were transplanted into BALB/c mice, some mice were given antibodies to deplete T cells. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
MEF2D protein acetylation and proteins that interact with MEF2D were identified by co-immunoprecipitation and pull-down assays. H22 cells, with MEF2D knockout or without (controls) were transplanted into BALB/c mice, some mice were given antibodies to deplete T cells. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene