About Enhancer
| Enhancer ID: | E_02812 |
| Species: | Mus musculus |
| Position : | chr5:141952000-141952800   |
| Year: | 2016 |
| Title: | Foxd3 Promotes Exit from Naive Pluripotency through Enhancer Decommissioning and Inhibits Germline Specification. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 26748758 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | Mouse embryonic stem cells (LF2 strain) |
| Cell Type: | mouse embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | Enhancer activity assay, TF binding assay, Functional perturbation assay, Enhancer decommissioning assay |
| High Throughput Method: | ChIP-seq; Strand-specific RNA-seq |
| Low Throughput Method: | GFP reporter assay; ChIP-RT-qPCR; RT-RT-qPCR; Co-immunoprecipitation; Western blot; EpiLC differentiation assay; Alkaline phosphatase staining |
About Target Gene
| Target gene : | Tbx3 |
About TF
| TF name : | Nanog |
About Regulatory State
| Regulatory State : | Repressed |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Nanog | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Nanog | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Nanog | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
Enhancer Associated Network
Expression of Target Genes for the Enhancer