About Enhancer
| Enhancer ID: | E_02639 |
| Species: | Mus musculus |
| Position : | chr4:147333142-147344738   |
| Year: | 2021 |
| Title: | Genetic Dissection of a Super Enhancer Controlling the Nppa-Nppb Cluster in the Heart. |
| Genome Build: | mm9 |
| Enhancer Type: | Super-enhancer |
| Condition: | Normal |
| PMID: | 33107387 |
| Tissue: | heart |
| Tissue Ontology ID: | UBERON:0000948 |
| Cell Source: | mouse ventricular myocardium |
| Cell Type: | cardiac muscle cell |
| Cell Ontology ID: | CL:0000746 |
| Experiment Type: | In vivo enhancer-cluster genetic dissection; chromatin topology analysis; enhancer-subdomain perturbation; Endogenous enhancer perturbation; In vivo functional validation |
| High Throughput Method: | 4C-seq; RNA-seq; H3K27ac CUT&RUN; epigenomic enhancer-cluster analysis |
| Low Throughput Method: | CRISPR/Cas9 deletion of RE1 and constituent RE1a/RE1b regulatory elements; RT-qPCR; in situ expression analysis; myocardial infarction and hypertrophic-stress phenotyping; Start/End denote the approximately 11.6-kb RE1 functional enhancer-cluster domain rather than a single constituent enhancer; CRISPR-Cas9 endogenous deletion/editing; Mouse genetic deletion/knock-in; In vivo phenotyping |
About Target Gene
| Target gene : | Nppb |
About TF
| TF name : | Nkx2-5 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Nkx2-5 | Physiological factors | reactome | 12 |
| Nkx2-5 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Nkx2-5 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| Nkx2-5 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
Enhancer Associated Network
Overlapping Enhancers (mm9)
5 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02636 | chr4:147333142-147344738 | heart | cardiac muscle cell | NA |
| E_02637 | chr4:147333142-147344738 | heart | cardiac muscle cell | NA |
| E_02638 | chr4:147333142-147344738 | heart | cardiac muscle cell | NA |
| E_02640 | chr4:147333142-147344738 | heart | cardiac muscle cell | NA |
| E_02641 | chr4:147333142-147344738 | heart | cardiac muscle cell | NA |
Expression of Target Genes for the Enhancer