About Enhancer
| Enhancer ID: | E_02467 |
| Species: | Mus musculus |
| Position : | chr7:45416017-45418912   |
| Year: | 2024 |
| Title: | An i-motif-regulated enhancer, eRNA and adjacent lncRNA affect Lhb expression through distinct mechanisms in a sex-specific context. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 39158745 |
| Tissue: | pituitary gland |
| Tissue Ontology ID: | UBERON:0000007 |
| Cell Source: | LbetaT2 |
| CVCL ID: | CVCL_0398 |
| Cell Type: | gonadotrope |
| Experiment Type: | Enhancer activity assay; Chromatin interaction assay; Chromatin regulator binding assay; Functional perturbation assay; Non-canonical DNA structure assay |
| High Throughput Method: | snATAC-seq reanalysis; UMI-4C-seq; LC-MS/MS proteomics |
| Low Throughput Method: | dCas9-KRAB CRISPRi; dCas9-VP64 CRISPRa; H3K4me1 ChIP-qPCR; H3K4me3 ChIP-qPCR; Hmgb2 ChIP-qPCR; BG4 ChIP-qPCR; iMab ChIP-qPCR; shRNA-mediated Hmgb2 knockdown; shRNA-mediated eRNA2 knockdown; RT-qPCR; circular dichroism; fluorescence binding assay; dCas9/FLAG locus-specific chromatin pull-down |
About Target Gene
| Target gene : | Lhb |
About TF
| TF name : | Hmgb2 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Hmgb2 | Activation of DNA fragmentation factor | reactome | 13 |
Enhancer Associated Network
Expression of Target Genes for the Enhancer