About Enhancer
| Enhancer ID: | E_02229 |
| Species: | Mus musculus |
| Position : | chr18:61241648-61241998   |
| Year: | 2019 |
| Title: | Deletion of a Csf1r enhancer selectively impacts CSF1R expression and development of tissue macrophage populations. |
| Genome Build: | mm39 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 31324781 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | embryonic macrophages |
| Cell Type: | macrophage |
| Cell Ontology ID: | CL:0000235 |
| Experiment Type: | Endogenous enhancer deletion; tissue-specific macrophage-development phenotyping; enhancer/TF chromatin profiling; Endogenous enhancer perturbation; In vivo functional validation |
| High Throughput Method: | ATAC-seq; ChIP-seq; RNA expression profiling/microarray |
| Low Throughput Method: | CRISPR/genomic FIRE deletion; transgenic reporter; flow cytometry; immunohistochemistry; mESC macrophage differentiation; Mouse genetic deletion/knock-in |
About Target Gene
| Target gene : | Csf1r |
About TF
| TF name : | Spi1 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Spi1 | C-MYB transcription factor network | pid | 87 |
| Spi1 | ERK cascade ( FGF8 signaling (Mouse) ) | inoh | 17 |
| Spi1 | ERK cascade ( FGF8 signaling (Xenopus) ) | inoh | 17 |
| Spi1 | FGF8 signaling pathway(Mouse) ( FGF8 signaling (Mouse) ) | inoh | 20 |
| Spi1 | FGF8 signaling pathway(Xenopus) ( FGF8 signaling (Xenopus) ) | inoh | 18 |
| Spi1 | Glucocorticoid receptor regulatory network | pid | 85 |
| Spi1 | IL4-mediated signaling events | pid | 66 |
| Spi1 | IL4 | netpath | 75 |
| Spi1 | Interleukin signaling pathway | panther | 86 |
| Spi1 | RANKL | netpath | 84 |
| Spi1 | Regulation of retinoblastoma protein | pid | 67 |
| Spi1 | Validated targets of C-MYC transcriptional repression | pid | 63 |
| Spi1 | Pathways in cancer | kegg | 321 |
| Spi1 | Acute myeloid leukemia | kegg | 53 |
| Spi1 | Hs_RANKL-RANK_(Receptor_activator_of_NFKB_(ligand))_Signaling_Pathway_WP2018_90016 | wikipathways | 26 |
Enhancer Associated Network
Overlapping Enhancers (mm39)
6 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02230 | chr18:61241648-61241998 | heart | macrophage | NA |
| E_02231 | chr18:61241648-61241998 | kidney | macrophage | NA |
| E_02232 | chr18:61241648-61241998 | embryo | macrophage | NA |
| E_02233 | chr18:61241648-61241998 | peritoneal cavity | macrophage | NA |
| E_02234 | chr18:61241648-61241998 | skin of body | macrophage | NA |
| E_02250 | chr18:61241648-61241998 | bone marrow | monocyte/myeloid progenitor | NA |
Expression of Target Genes for the Enhancer