About Enhancer
| Enhancer ID: | E_02175 |
| Species: | Mus musculus |
| Position : | chrX:103578000-103588000   |
| Year: | 2023 |
| Title: | GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 37932452 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | GATA6-inducible mouse embryonic stem cells |
| Cell Type: | embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | RE79 long-range enhancer functional validation; TF binding assay; Functional perturbation assay; database working interval; Endogenous enhancer perturbation; In vivo functional validation |
| High Throughput Method: | CUT&Tag; CRISPRa screen; RNA-seq; ATAC-seq; pooled CRISPRa screen |
| Low Throughput Method: | FIREWACh lentiviral enhancer reporter assay; CRISPRi; GATA6 induction; qRT-PCR; RNA-FISH; zygotic CRISPR/Cas9 RE79/RE97 deletion; CRISPRa; Mouse genetic deletion/knock-in |
About Target Gene
| Target gene : | Xist |
About TF
| TF name : | Gata6 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Gata6 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| Gata6 | Notch-mediated HES/HEY network | pid | 48 |
| Gata6 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Gata6 | Surfactant metabolism | reactome | 30 |
| Gata6 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02226 | chrX:103578000-103588000 | embryo | extraembryonic endoderm cell | NA |
Expression of Target Genes for the Enhancer