About Enhancer
| Enhancer ID: | E_02102 |
| Species: | Mus musculus |
| Position : | chr15:62092610-62093180   |
| Year: | 2024 |
| Title: | Regulation of Myc transcription by an enhancer cluster dedicated to pluripotency and early embryonic expression. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 38729993 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | MycGFP/GFP mESC-derived EpiLCs (2 days N2B27+FGF2/Activin A) and E6.0-E6.5 post-implantation epiblast |
| Cell Type: | epiblast cell |
| Cell Ontology ID: | CL:0000352 |
| Experiment Type: | Enhancer activity assay; TF binding evidence; Chromatin interaction assay; Functional perturbation assay; Endogenous enhancer perturbation; In vivo functional validation |
| High Throughput Method: | 4C-seq; public ChIP-seq meta-analysis; public PLAC-seq; public Hi-C |
| Low Throughput Method: | CRISPR-Cas9 FPE/enhancer-7-3 deletion; 32-bp OTX2 ChIP-peak-centered deletion; GFP-MYC flow cytometry; confocal immunofluorescence; in vivo enhancer knockout in MycGFP/GFP embryos; PCR and sequencing; CRISPR-Cas9 endogenous deletion/editing; Mouse genetic deletion/knock-in |
About Target Gene
| Target gene : | Myc |
About TF
| TF name : | Otx2 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer Associated Network
Expression of Target Genes for the Enhancer