About Enhancer
| Enhancer ID: | E_02099 |
| Species: | Mus musculus |
| Position : | chr15:62009732-62013089   |
| Year: | 2024 |
| Title: | Regulation of Myc transcription by an enhancer cluster dedicated to pluripotency and early embryonic expression. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 38729993 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | naive pluripotency) and E3.5 blastocyst epiblast |
| Cell Type: | embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Chromatin interaction assay; Functional perturbation assay; Endogenous enhancer perturbation; In vivo functional validation |
| High Throughput Method: | 4C-seq; public ChIP-seq meta-analysis; public PLAC-seq; public Hi-C |
| Low Throughput Method: | CRISPR-Cas9 NPE/enhancer-2 deletion; CRISPR-Cas9 FOXD3-binding-site deletion; GFP-MYC flow cytometry; confocal immunofluorescence; in vivo enhancer knockout in MycGFP/GFP embryos; PCR and sequencing; CRISPR-Cas9 endogenous deletion/editing; Mouse genetic deletion/knock-in |
About Target Gene
| Target gene : | Myc |
About TF
| TF name : | Foxd3 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Foxd3 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Foxd3 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
7 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01398 | chr15:61825000-62125000 | spleen | mature B cell | NA |
| E_02040 | chr15:61958143-62188886 | embryo | mouse embryonic stem cell | NA |
| E_02096 | chr15:62009732-62013089 | embryo | embryonic stem cell | NA |
| E_02097 | chr15:62009732-62013089 | embryo | embryonic stem cell | NA |
| E_02098 | chr15:62009732-62013089 | embryo | embryonic stem cell | NA |
| E_02100 | chr15:62009732-62013089 | embryo | embryonic stem cell | NA |
| E_02101 | chr15:62009732-62013089 | embryo | embryonic stem cell | NA |
Expression of Target Genes for the Enhancer