About Enhancer
| Enhancer ID: | E_02028 |
| Species: | Mus musculus |
| Position : | chr14:99260419-99393893   |
| Year: | 2025 |
| Title: | Klf5-adjacent super-enhancer functions as a 3D genome structure-dependent transcriptional driver to safeguard ESC identity. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 40592854 |
| Tissue: | embryonic tissue |
| Tissue Ontology ID: | UBERON:0005291 |
| Cell Source: | ES-R1 |
| CVCL ID: | CVCL_2167 |
| Cell Type: | mouse embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | Enhancer activity assay, TF binding assay, Chromatin interaction assay, Functional perturbation assay |
| High Throughput Method: | ChIP-seq; RNA-seq; Hi-C, 4C-seq, scRNA-seq; ATAC-seq; single-cell RNA-seq; Hi-C |
| Low Throughput Method: | CRISPR Knockout; CRISPRa, qRT-PCR; Western blot, 3C-PCR |
About Target Gene
| Target gene : | Klf5 |
About TF
| TF name : | Sox2 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sox2 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sox2 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Sox2 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Sox2 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| Sox2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| Sox2 | Hs_Dopaminergic_Neurogenesis_WP2855_87239 | wikipathways | 19 |
| Sox2 | Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 | wikipathways | 12 |
| Sox2 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
| Sox2 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
7 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02143 | chr14:99319699-99358748 | embryonic stem cell | embryonic stem cell | NA |
| E_02144 | chr14:99319699-99358748 | embryonic stem cell | embryonic stem cell | NA |
| E_02145 | chr14:99319699-99358748 | embryonic stem cell | embryonic stem cell | NA |
| E_02146 | chr14:99319699-99358748 | embryonic stem cell | embryonic stem cell | NA |
| E_01542 | chr14:99338943-99341862 | embryo | embryonic stem cell | NA |
| E_01562 | chr14:99338943-99341862 | embryo | primordial germ cell-like cell | NA |
| E_01563 | chr14:99338943-99341862 | embryo | epiblast-like cell | NA |
Expression of Target Genes for the Enhancer