About Enhancer
| Enhancer ID: | E_01_0987 |
| Species: | human |
| Position : | chr11:1980587-1982587   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Initiating cystic carcinoma lung metastases |
| Pubmed ID: | 32098781 |
| Enhancer experiment: | RT-qPCR, invasion migration assay of transfected cells, Western blot, rip SEQ, chip SEQ, rna-fish and immunofluorescence, flow cytometry |
| Enhancer experiment description: | MRPL23-AS1 increased the binding of EZH2 and H3K27me3 on the E-cadherin promoter region. Moreover, MRPL23-AS1 levels were higher in exosomes isolated from the blood plasma of SACC patients, and exosomal MRPL23-AS1 affected pulmonary microvascular endothelial cells in an 'exosomecrine' manner. |
About Target gene
| Target gene : | MRPL23-AS1 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | MRPL23-AS1 increased the binding of EZH2 and H3K27me3 on the E-cadherin promoter region. Moreover, MRPL23-AS1 levels were higher in exosomes isolated from the blood plasma of SACC patients, and exosomal MRPL23-AS1 affected pulmonary microvascular endothelial cells in an 'exosomecrine' manner. |
About TF
| TF name : | -- |
| TF experiment: | RT-QPCR????????????Western blot?RIP-seq?CHIP-seq?RNA-FISH??????????? |
| TF experiment description: | MRPL23-AS1 increased the binding of EZH2 and H3K27me3 on the E-cadherin promoter region. Moreover, MRPL23-AS1 levels were higher in exosomes isolated from the blood plasma of SACC patients, and exosomal MRPL23-AS1 affected pulmonary microvascular endothelial cells in an 'exosomecrine' manner. |
About Function
| Enhancer function : | MRPL23-AS1 increased the binding of EZH2 and H3K27me3 on the E-cadherin promoter region. Moreover, MRPL23-AS1 levels were higher in exosomes isolated from the blood plasma of SACC patients, and exosomal MRPL23-AS1 affected pulmonary microvascular endothelial cells in an 'exosomecrine' manner. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
MRPL23-AS1 increased the binding of EZH2 and H3K27me3 on the E-cadherin promoter region. Moreover, MRPL23-AS1 levels were higher in exosomes isolated from the blood plasma of SACC patients, and exosomal MRPL23-AS1 affected pulmonary microvascular endothelial cells in an 'exosomecrine' manner. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene