About Enhancer
| Enhancer ID: | E_01_0798 |
| Species: | human |
| Position : | chr4:109910279-109912279   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Colorectal adenocarcinoma |
| Pubmed ID: | 30022673 |
| Enhancer experiment: | NF - ? B assay, flow cytometry, statistical analysis, immunofluorescence staining |
| Enhancer experiment description: | The immortalised cells were routinely propagated in Opti-MEM, supplemented with 4% v/v fetal bovine serum (FBS), 2mM L-glutamine, 2mM GlutaMAX, 10mM 4-(2-hydroxyethyl)-1-piperazineethanesul- phonic acid (HEPES), 20ng/ml Epidermal Growth Factor (EGF), 10 g/ml insulin, and 150nM hydrocor- tisone, and were incubated at 32 C, 6% v/v CO 2 in a humidified atmosphere. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | The immortalised cells were routinely propagated in Opti-MEM, supplemented with 4% v/v fetal bovine serum (FBS), 2mM L-glutamine, 2mM GlutaMAX, 10mM 4-(2-hydroxyethyl)-1-piperazineethanesul- phonic acid (HEPES), 20ng/ml Epidermal Growth Factor (EGF), 10 g/ml insulin, and 150nM hydrocor- tisone, and were incubated at 32 C, 6% v/v CO 2 in a humidified atmosphere. |
About TF
| TF name : | EGF(HOMG4,URG) |
| TF experiment: | NF-?B assay,?????,????,?????? |
| TF experiment description: | The immortalised cells were routinely propagated in Opti-MEM, supplemented with 4% v/v fetal bovine serum (FBS), 2mM L-glutamine, 2mM GlutaMAX, 10mM 4-(2-hydroxyethyl)-1-piperazineethanesul- phonic acid (HEPES), 20ng/ml Epidermal Growth Factor (EGF), 10 g/ml insulin, and 150nM hydrocor- tisone, and were incubated at 32 C, 6% v/v CO 2 in a humidified atmosphere. |
About Function
| Enhancer function : | The immortalised cells were routinely propagated in Opti-MEM, supplemented with 4% v/v fetal bovine serum (FBS), 2mM L-glutamine, 2mM GlutaMAX, 10mM 4-(2-hydroxyethyl)-1-piperazineethanesul- phonic acid (HEPES), 20ng/ml Epidermal Growth Factor (EGF), 10 g/ml insulin, and 150nM hydrocor- tisone, and were incubated at 32 C, 6% v/v CO 2 in a humidified atmosphere. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
The immortalised cells were routinely propagated in Opti-MEM, supplemented with 4% v/v fetal bovine serum (FBS), 2mM L-glutamine, 2mM GlutaMAX, 10mM 4-(2-hydroxyethyl)-1-piperazineethanesul- phonic acid (HEPES), 20ng/ml Epidermal Growth Factor (EGF), 10 g/ml insulin, and 150nM hydrocor- tisone, and were incubated at 32 C, 6% v/v CO 2 in a humidified atmosphere. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene