About Enhancer
| Enhancer ID: | E_01_0679 |
| Species: | human |
| Position : | chr8:144288777-144290777   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Cellular upregulation of the proteotoxic stress response |
| Pubmed ID: | 30307772 |
| Enhancer experiment: | Chromatin immunoprecipitation,Chromatin conformation capture assay,Semi-quantitative RT-PCR and quantitative RT-PCR,EMSA,MTT cytotoxicity assay,RNA transfection, Immunoprecipitation,Immunoblot |
| Enhancer experiment description: | We show here that theTNF-agene, apleiotropicNkBS inducer, is a direct target of heat shock factor 1 (HSF1). HumanHSF1 drives this process by assembling a multiprotein activation complex at a heat shock element (HSE) located at the 39-UTR of the TNF-a gene (HSE5). |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | We show here that theTNF-agene, apleiotropicNkBS inducer, is a direct target of heat shock factor 1 (HSF1). HumanHSF1 drives this process by assembling a multiprotein activation complex at a heat shock element (HSE) located at the 39-UTR of the TNF-a gene (HSE5). |
About TF
| TF name : | HSF1(HSTF1) |
| TF experiment: | Chromatin immunoprecipitation,Chromatin conformation capture assay,Semi-quantitative RT-PCR and quantitative RT-PCR,EMSA,MTT cytotoxicity assay,RNA transfection, Immunoprecipitation,Immunoblot |
| TF experiment description: | We show here that theTNF-agene, apleiotropicNkBS inducer, is a direct target of heat shock factor 1 (HSF1). HumanHSF1 drives this process by assembling a multiprotein activation complex at a heat shock element (HSE) located at the 39-UTR of the TNF-a gene (HSE5). |
About Function
| Enhancer function : | We show here that theTNF-agene, apleiotropicNkBS inducer, is a direct target of heat shock factor 1 (HSF1). HumanHSF1 drives this process by assembling a multiprotein activation complex at a heat shock element (HSE) located at the 39-UTR of the TNF-a gene (HSE5). |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
We show here that theTNF-agene, apleiotropicNkBS inducer, is a direct target of heat shock factor 1 (HSF1). HumanHSF1 drives this process by assembling a multiprotein activation complex at a heat shock element (HSE) located at the 39-UTR of the TNF-a gene (HSE5). |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| HSF1 | Attenuation phase | reactome | 28 |
| HSF1 | HSF1-dependent transactivation | reactome | 34 |
| HSF1 | HSF1 activation | reactome | 31 |
| HSF1 | p73 transcription factor network | pid | 80 |
| HSF1 | Regulation of HSF1-mediated heat shock response | reactome | 82 |
| HSF1 | Hs_DNA_IR-Double_Strand_Breaks_(DSBs)_and_cellular_response_via_ATM_WP3959_91367 | wikipathways | 50 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene