About Enhancer
| Enhancer ID: | E_01_0647 |
| Species: | human |
| Position : | chr12:2854445-2856445   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Osteosarcoma |
| Pubmed ID: | 30387181 |
| Enhancer experiment: | cell transfection,Quantitative real?time polymerase Dual?luciferase reporter assay,Western blo,chain reaction,Cell viability assay,Cell colony formation assay,Cell invasion assay |
| Enhancer experiment description: | Downregulation of miR 134 attenuated DADS induced antitumor capacity. Further bioinformatics prediction analysis revealed that the 3 untranslated region (3 UTR) of Forkhead Box M1 (FOXM1) harbored miR 134 binding sites, and overexpression of miR 134 repressed the luciferase activity of the reporting vector containing FOXM1 3 UTR. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Downregulation of miR 134 attenuated DADS induced antitumor capacity. Further bioinformatics prediction analysis revealed that the 3 untranslated region (3 UTR) of Forkhead Box M1 (FOXM1) harbored miR 134 binding sites, and overexpression of miR 134 repressed the luciferase activity of the reporting vector containing FOXM1 3 UTR. |
About TF
| TF name : | FOXM1(FKHL16A,FOXM1B,FOXM1C,HFH-11,HFH11,HNF-3,INS-1,MPHOSPH2,MPP-2,MPP2,PIG29,TRIDENT,FOXM1) |
| TF experiment: | cell transfection,Quantitative real?time polymerase Dual?luciferase reporter assay,Western blo,chain reaction,Cell viability assay,Cell colony formation assay,Cell invasion assay |
| TF experiment description: | Downregulation of miR 134 attenuated DADS induced antitumor capacity. Further bioinformatics prediction analysis revealed that the 3 untranslated region (3 UTR) of Forkhead Box M1 (FOXM1) harbored miR 134 binding sites, and overexpression of miR 134 repressed the luciferase activity of the reporting vector containing FOXM1 3 UTR. |
About Function
| Enhancer function : | Downregulation of miR 134 attenuated DADS induced antitumor capacity. Further bioinformatics prediction analysis revealed that the 3 untranslated region (3 UTR) of Forkhead Box M1 (FOXM1) harbored miR 134 binding sites, and overexpression of miR 134 repressed the luciferase activity of the reporting vector containing FOXM1 3 UTR. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Downregulation of miR 134 attenuated DADS induced antitumor capacity. Further bioinformatics prediction analysis revealed that the 3 untranslated region (3 UTR) of Forkhead Box M1 (FOXM1) harbored miR 134 binding sites, and overexpression of miR 134 repressed the luciferase activity of the reporting vector containing FOXM1 3 UTR. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| FOXM1 | Cyclin A/B1 associated events during G2/M transition | reactome | 22 |
| FOXM1 | FOXM1 transcription factor network | pid | 43 |
| FOXM1 | Polo-like kinase mediated events | reactome | 16 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene