About Enhancer
| Enhancer ID: | E_01_0637 |
| Species: | human |
| Position : | chr3:189628920-189630920   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Squamous subtype |
| Pubmed ID: | 30428345 |
| Enhancer experiment: | Plasmid Construction?Lentiviral Production and Infection?In Vitro Phenotypic Assays?CRISPR-Based Targeting?shRNA targeting in BxPC3 cells and hF3 organoids?In Vivo Transplantation Experiments?Histology and Immunohistochemistry?Cell Lysate Preparation for Western Blot Analysis?RNA Extraction and RT-PCR?RNA-seq Library Construction?ChIP and ChIP-Seq Library Construction?RNA-Seq Data Analysis?ChIP-Seq Analysis |
| Enhancer experiment description: | The transcription factor TP63 (DNp63) is sufficient to install and sustain the enhancer landscape and transcriptional signature of the squamous lineage in human PDA cells. We also demonstrate that TP63-driven enhancer reprogramming promotes aggressive tumor phenotypes, including enhanced cell motility and invasion, and an accelerated growth of primary PDA tumors and metastases in vivo. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | The transcription factor TP63 (DNp63) is sufficient to install and sustain the enhancer landscape and transcriptional signature of the squamous lineage in human PDA cells. We also demonstrate that TP63-driven enhancer reprogramming promotes aggressive tumor phenotypes, including enhanced cell motility and invasion, and an accelerated growth of primary PDA tumors and metastases in vivo. |
About TF
| TF name : | TP63(AIS,B(p51A),B(p51B),EEC3,KET,LMS,NBP,OFC8,RHS,SHFM4,TP53CP,TP53L,TP73L,p40,p51,p53CP,p63,p73H,p73L) |
| TF experiment: | Plasmid Construction?Lentiviral Production and Infection?In Vitro Phenotypic Assays?CRISPR-Based Targeting?shRNA targeting in BxPC3 cells and hF3 organoids?In Vivo Transplantation Experiments?Histology and Immunohistochemistry?Cell Lysate Preparation for Western Blot Analysis?RNA Extraction and RT-PCR?RNA-seq Library Construction?ChIP and ChIP-Seq Library Construction?RNA-Seq Data Analysis?ChIP-Seq Analysis |
| TF experiment description: | The transcription factor TP63 (DNp63) is sufficient to install and sustain the enhancer landscape and transcriptional signature of the squamous lineage in human PDA cells. We also demonstrate that TP63-driven enhancer reprogramming promotes aggressive tumor phenotypes, including enhanced cell motility and invasion, and an accelerated growth of primary PDA tumors and metastases in vivo. |
About Function
| Enhancer function : | The transcription factor TP63 (DNp63) is sufficient to install and sustain the enhancer landscape and transcriptional signature of the squamous lineage in human PDA cells. We also demonstrate that TP63-driven enhancer reprogramming promotes aggressive tumor phenotypes, including enhanced cell motility and invasion, and an accelerated growth of primary PDA tumors and metastases in vivo. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
The transcription factor TP63 (DNp63) is sufficient to install and sustain the enhancer landscape and transcriptional signature of the squamous lineage in human PDA cells. We also demonstrate that TP63-driven enhancer reprogramming promotes aggressive tumor phenotypes, including enhanced cell motility and invasion, and an accelerated growth of primary PDA tumors and metastases in vivo. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene