About Enhancer
| Enhancer ID: | E_01_0636 |
| Species: | human |
| Position : | chr19:16321911-16323911   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Coronary artery disease and ischemic stroke |
| Pubmed ID: | 30429326 |
| Enhancer experiment: | ATAC-Seq?Genome-wide association studies (GWAS)?ChIP-seq? |
| Enhancer experiment description: | H3K27ac/H3K4me2 ChIP with whole-genome sequencing (ChIP-seq), and CRISPR interference in human aortic endothelial cells (HAECs),CAD/IS-protective allele at rs17114036 creates a binding site for transcription factor Krppel-like factor 2 (KLF2), which increases the enhancer activity under unidirectional flow. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | H3K27ac/H3K4me2 ChIP with whole-genome sequencing (ChIP-seq), and CRISPR interference in human aortic endothelial cells (HAECs),CAD/IS-protective allele at rs17114036 creates a binding site for transcription factor Krppel-like factor 2 (KLF2), which increases the enhancer activity under unidirectional flow. |
About TF
| TF name : | KLF2(LKLF) |
| TF experiment: | ATAC-Seq?Genome-wide association studies (GWAS)?ChIP-seq? |
| TF experiment description: | H3K27ac/H3K4me2 ChIP with whole-genome sequencing (ChIP-seq), and CRISPR interference in human aortic endothelial cells (HAECs),CAD/IS-protective allele at rs17114036 creates a binding site for transcription factor Krppel-like factor 2 (KLF2), which increases the enhancer activity under unidirectional flow. |
About Function
| Enhancer function : | H3K27ac/H3K4me2 ChIP with whole-genome sequencing (ChIP-seq), and CRISPR interference in human aortic endothelial cells (HAECs),CAD/IS-protective allele at rs17114036 creates a binding site for transcription factor Krppel-like factor 2 (KLF2), which increases the enhancer activity under unidirectional flow. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
H3K27ac/H3K4me2 ChIP with whole-genome sequencing (ChIP-seq), and CRISPR interference in human aortic endothelial cells (HAECs),CAD/IS-protective allele at rs17114036 creates a binding site for transcription factor Krppel-like factor 2 (KLF2), which increases the enhancer activity under unidirectional flow. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| KLF2 | Hs_White_fat_cell_differentiation_WP3946_90940 | wikipathways | 30 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene