About Enhancer
| Enhancer ID: | E_01_0633 |
| Species: | human |
| Position : | chr4:108044811-108046811   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Colorectal cancer |
| Pubmed ID: | 30446587 |
| Enhancer experiment: | Luciferase reporter assay, Western blot,immunofluorescence analysis,histopathological analysis ,short tandem repeat profiling analysis, cell proliferation assay, MTT assay,FACS analysis |
| Enhancer experiment description: | Niclosamide blocks the transcription of DCLK1-B by interrupting the binding of LEF1 to DCLK1-B promoter. DCLK1-B depletion impairs cancer stemness resulting in reduced survival potential and increased apoptosis, thus sensitizing CRC to chemoradiation. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Niclosamide blocks the transcription of DCLK1-B by interrupting the binding of LEF1 to DCLK1-B promoter. DCLK1-B depletion impairs cancer stemness resulting in reduced survival potential and increased apoptosis, thus sensitizing CRC to chemoradiation. |
About TF
| TF name : | LEF1(LEF-1,TCF10,TCF1ALPHA,TCF7L3) |
| TF experiment: | Luciferase reporter assay, Western blot,immunofluorescence analysis,histopathological analysis ,short tandem repeat profiling analysis, cell proliferation assay, MTT assay,FACS analysis |
| TF experiment description: | Niclosamide blocks the transcription of DCLK1-B by interrupting the binding of LEF1 to DCLK1-B promoter. DCLK1-B depletion impairs cancer stemness resulting in reduced survival potential and increased apoptosis, thus sensitizing CRC to chemoradiation. |
About Function
| Enhancer function : | Niclosamide blocks the transcription of DCLK1-B by interrupting the binding of LEF1 to DCLK1-B promoter. DCLK1-B depletion impairs cancer stemness resulting in reduced survival potential and increased apoptosis, thus sensitizing CRC to chemoradiation. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Niclosamide blocks the transcription of DCLK1-B by interrupting the binding of LEF1 to DCLK1-B promoter. DCLK1-B depletion impairs cancer stemness resulting in reduced survival potential and increased apoptosis, thus sensitizing CRC to chemoradiation. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene