About Enhancer

Enhancer ID: E_01_0595
Species: human
Position : chr4:176680985-176682985
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: --
Pubmed ID:  32442411
Enhancer experiment: High-throughput Seq?eQTL Analysis?ChIP-seq?ATAC-seq?
Enhancer experiment description: Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.

About Target gene

Target gene : FGD6,KIF26B
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.;Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.;Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.

About TF

TF name : VEGFC
TF experiment: High-throughput Seq?eQTL Analysis?ChIP-seq?ATAC-seq?
TF experiment description: Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.;Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.;Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.

About Function

Enhancer function : Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 Using CRISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we validated candidate enhancer variants located up to 750 kb from their target genes, VEGFC, FGD6, and KIF26B.

About SNP

SNP ID: rs12028528,rs7975658,rs6825977,rs17114036

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
VEGFC Alpha9 beta1 integrin signaling events pid 25
VEGFC Heterotrimeric GPCR signaling pathway (through G alpha i and pertussis toxin) ( GPCR signaling (pertussis toxin) ) inoh 228
VEGFC Heterotrimeric GPCR signaling pathway (through G alpha q, PLC beta and ERK cascade) ( GPCR signaling (G alpha q) ) inoh 239
VEGFC Heterotrimeric GPCR signaling pathway (through G alpha s ACs Epac BRaf and ERKcascade) ( GPCR signaling (G alpha s, Epac and ERK) ) inoh 237
VEGFC Heterotrimeric GPCR signaling pathway (through_G alpha s_ACs_PKA_BRaf_and_ERKcascade)(canonical) ( GPCR signaling (G alpha s, PKA and ERK) ) inoh 227
VEGFC Heterotrimeric GTP-binding protein coupled receptor signaling pathway (through G alpha i, adenylate cyclase and cAMP) ( GPCR signaling (G alpha i) ) inoh 228
VEGFC Heterotrimeric GTP-binding protein coupled receptor signaling pathway (through_G_alpha_s,_cholera_toxin,_adenylate_cyclase_and_cAMP) ( GPCR signaling (cholera toxin) ) inoh 227
VEGFC Platelet degranulation reactome 107
VEGFC VEGF and VEGFR signaling network pid 10
VEGFC VEGF binds to VEGFR leading to receptor dimerization reactome 8
VEGFC VEGF ligand-receptor interactions reactome 5
VEGFC VEGF signaling pathway ( VEGF signaling pathway ) inoh 183
VEGFC VEGFR3 signaling in lymphatic endothelium pid 26
VEGFC Cytokine-cytokine receptor interaction kegg 213
VEGFC mTOR signaling pathway kegg 50
VEGFC Focal adhesion kegg 197
VEGFC Pathways in cancer kegg 321
VEGFC Renal cell carcinoma kegg 65
VEGFC Pancreatic cancer kegg 70
VEGFC Bladder cancer kegg 38
VEGFC Hs_miRNAs_involved_in_DNA_damage_response_WP1545_84697 wikipathways 30

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs