About Enhancer
| Enhancer ID: | E_01_0572 |
| Species: | human |
| Position : | chr5:51380554-51382554   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Cancer |
| Pubmed ID: | 32320655 |
| Enhancer experiment: | GRO-seq, RNA-seq?RNA-seq?ChIP-seq?Western blotting?qRT_x005f_x0002_PCR?Immunofluorescent imaging?flow cytometry |
| Enhancer experiment description: | Using a PageRank-based algorithm, we predict super-enhancer regulator ISL1 as a driver of this plasticity and validate this prediction by using CRISPR/dCas9-KRAB inhibition (CRISPRi) and CRISPR/dCas9-VP64 activation (CRISPRa) tools. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Using a PageRank-based algorithm, we predict super-enhancer regulator ISL1 as a driver of this plasticity and validate this prediction by using CRISPR/dCas9-KRAB inhibition (CRISPRi) and CRISPR/dCas9-VP64 activation (CRISPRa) tools. |
About TF
| TF name : | ISL1(ISLET1,Isl-1) |
| TF experiment: | GRO-seq, RNA-seq?RNA-seq?ChIP-seq?Western blotting?qRT_x005f_x0002_PCR?Immunofluorescent imaging?flow cytometry |
| TF experiment description: | Using a PageRank-based algorithm, we predict super-enhancer regulator ISL1 as a driver of this plasticity and validate this prediction by using CRISPR/dCas9-KRAB inhibition (CRISPRi) and CRISPR/dCas9-VP64 activation (CRISPRa) tools. |
About Function
| Enhancer function : | Using a PageRank-based algorithm, we predict super-enhancer regulator ISL1 as a driver of this plasticity and validate this prediction by using CRISPR/dCas9-KRAB inhibition (CRISPRi) and CRISPR/dCas9-VP64 activation (CRISPRa) tools. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Using a PageRank-based algorithm, we predict super-enhancer regulator ISL1 as a driver of this plasticity and validate this prediction by using CRISPR/dCas9-KRAB inhibition (CRISPRi) and CRISPR/dCas9-VP64 activation (CRISPRa) tools. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| ISL1 | Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP) | reactome | 13 |
| ISL1 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| ISL1 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene