About Enhancer
| Enhancer ID: | E_01_0517 |
| Species: | human |
| Position : | chr19:6658582-6660582   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Skin pigmentation disturbances |
| Pubmed ID: | 29730385 |
| Enhancer experiment: | RNA-seq,transfection,RT-qPCR,Cell proliferation assay,Western blot, |
| Enhancer experiment description: | To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
About Target gene
| Target gene : | TNFSF14 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
About TF
| TF name : | -- |
| TF experiment: | RNA-seq,transfection,RT-qPCR,Cell proliferation assay,Western blot, |
| TF experiment description: | To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
About Function
| Enhancer function : | To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene